Non-Invasive Sperm Selection for In Vitro Fertilization

Ashok Agarwal, PhD, HCLD (ABB), ELD (ACE), Lerner College of Medicine, Andrology Center, Case Western Reserve University, Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USAEdson Borges Jr., MD, PhD, Fertility - Centro de Fertilização Assistida, Associação Instituto Sapientiae - Centro de Estudos e Pesquisa em Reprodução Assistida, São Paulo, BrazilAmanda S. Setti, BSc, Fertility - Centro de Fertilização Assistida, Associação Instituto Sapientiae - Centro de Estudos e Pesquisa em Reprodução Assistida, São Paulo, Brazil

• Male Infertility Laboratory Investigation: A Critical Analysis of the 2010 World Health Organization Semen Analysis Guidelines.- Intracytoplasmic Injection with Suboptimal Spermatozoa.- Novel Sperm Tests and Their Importance.- Sperm Selection Based on Surface Electrical Charge.- Microfluidics for Sperm Selection.- Sperm Binding to Zona Pellucida, Hyaluronic Acid Binding Assay and PICSI.- Non-Apoptopic Sperm Selection.- Motile Sperm Organelle Morphology Examination (MSOME).- Setup of Micromanipulator for Sperm Selection and Injection for IMSI.- Configuring the Microscope for Intracytoplasmic Morphology-Selected Sperm Injection (IMSI).- The Technical Background of Advanced IMSI Systems.- Sperm Vacuoles: Origin and Implications.- MSOME: Conventional Semen Analysis, Sperm Manipulation, and Cryopreservation.- MSOME and Sperm Chromatin Status.- MSOME and Sperm DNA Integrity: Biological and Clinical Considerations.- MSOME and Sperm Chromosomal Constitution.- Intracytoplasmic Morphologically Selected Sperm Injection (IMSI): Indications and Clinical Results.- Genomic and Proteomic Approaches in the Diagnosis of Male Infertility.

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