1. Dr Riyaz Ahmad Rather, PhDAssociate Professor, Department of Biotechnology, Wachemo University, Hossana, Ethiopia2. Prof. Dr. Subhas Chandra SahaProfessor, Department of Obstetrics and Gynecology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India

• Chapter 1 Evolution of Non-invasive Prenatal Screening (NIPS) Past to Present.- Chapter 2 Fetal origin circulating cell-free nucleic acids (cfNA) in maternal circulation and their clinical importance in fetal diagnosis.- Chapter 3 Cell-free fetal DNA (cffDNA): Genesis to clinical utility.- Chapter 4 Methods and protocols for extraction of cell-free fetal DNA (cffDNA) from the maternal circulation.- Chapter 5 Scope of cell- versus cell-free –based detection approach in Non-invasive Prenatal Screening (NIPS).- Chapter 6 Advanced genomic approaches in Non-invasive Prenatal Screening (NIPS) The road from conventional Karyotyping to Next-Gen Sequencing (NGS).- Chapter 7 Proteomic biomarkers of maternal plasma and their use in Non-invasive Prenatal Testing (NIPT).- Chapter 8 Non-invasive Prenatal Screening (NIPS) of chromosomal abnormalities.- Chapter 9 Non-invasive Prenatal Detection (NIPD) of fetal sex and RhD blood type.- Chapter 10 Detection of monogenic disorders using Non-invasive Prenatal Screening (NIPS).- Chapter 11 Screening of Placental dysfunction utilizing cell-free nucleic acids (cfNA) of maternal plasma.- Chapter 12 Non-invasive Prenatal Detection (NIPD) of Copy number variation (CNV) and sub-chromosomal variations.- Chapter 13 Circulation transcriptome of maternal plasma and its use in Non-invasive Prenatal Screening (NIPS).- Chapter 14 Challenges and ethical issues surrounding Non-invasive Prenatal Screening (NIPS).- Chapter 15 Implementation of Non-invasive Prenatal Screening (NIPS) in clinical practice: Comparison of developed versus developing countries.- Chapter 16 Sequencing: A promising path in the detection of fetal health.

1. Dr Riyaz Ahmad Rather, PhDAssociate Professor, Department of Biotechnology, Wachemo University, Hossana, Ethiopia2. Prof. Dr. Subhas Chandra SahaProfessor, Department of Obstetrics and Gynecology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India

• Chapter 1 Evolution of Non-invasive Prenatal Screening (NIPS) Past to Present.- Chapter 2 Fetal origin circulating cell-free nucleic acids (cfNA) in maternal circulation and their clinical importance in fetal diagnosis.- Chapter 3 Cell-free fetal DNA (cffDNA): Genesis to clinical utility.- Chapter 4 Methods and protocols for extraction of cell-free fetal DNA (cffDNA) from the maternal circulation.- Chapter 5 Scope of cell- versus cell-free –based detection approach in Non-invasive Prenatal Screening (NIPS).- Chapter 6 Advanced genomic approaches in Non-invasive Prenatal Screening (NIPS) The road from conventional Karyotyping to Next-Gen Sequencing (NGS).- Chapter 7 Proteomic biomarkers of maternal plasma and their use in Non-invasive Prenatal Testing (NIPT).- Chapter 8 Non-invasive Prenatal Screening (NIPS) of chromosomal abnormalities.- Chapter 9 Non-invasive Prenatal Detection (NIPD) of fetal sex and RhD blood type.- Chapter 10 Detection of monogenic disorders using Non-invasive Prenatal Screening (NIPS).- Chapter 11 Screening of Placental dysfunction utilizing cell-free nucleic acids (cfNA) of maternal plasma.- Chapter 12 Non-invasive Prenatal Detection (NIPD) of Copy number variation (CNV) and sub-chromosomal variations.- Chapter 13 Circulation transcriptome of maternal plasma and its use in Non-invasive Prenatal Screening (NIPS).- Chapter 14 Challenges and ethical issues surrounding Non-invasive Prenatal Screening (NIPS).- Chapter 15 Implementation of Non-invasive Prenatal Screening (NIPS) in clinical practice: Comparison of developed versus developing countries.- Chapter 16 Sequencing: A promising path in the detection of fetal health.

1. Dr Riyaz Ahmad Rather, PhDAssociate Professor, Department of Biotechnology, Wachemo University, Hossana, Ethiopia2. Prof. Dr. Subhas Chandra SahaProfessor, Department of Obstetrics and Gynecology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India

• Chapter 1 Evolution of Non-invasive Prenatal Screening (NIPS) Past to Present.- Chapter 2 Fetal origin circulating cell-free nucleic acids (cfNA) in maternal circulation and their clinical importance in fetal diagnosis.- Chapter 3 Cell-free fetal DNA (cffDNA): Genesis to clinical utility.- Chapter 4 Methods and protocols for extraction of cell-free fetal DNA (cffDNA) from the maternal circulation.- Chapter 5 Scope of cell- versus cell-free –based detection approach in Non-invasive Prenatal Screening (NIPS).- Chapter 6 Advanced genomic approaches in Non-invasive Prenatal Screening (NIPS) The road from conventional Karyotyping to Next-Gen Sequencing (NGS).- Chapter 7 Proteomic biomarkers of maternal plasma and their use in Non-invasive Prenatal Testing (NIPT).- Chapter 8 Non-invasive Prenatal Screening (NIPS) of chromosomal abnormalities.- Chapter 9 Non-invasive Prenatal Detection (NIPD) of fetal sex and RhD blood type.- Chapter 10 Detection of monogenic disorders using Non-invasive Prenatal Screening (NIPS).- Chapter 11 Screening of Placental dysfunction utilizing cell-free nucleic acids (cfNA) of maternal plasma.- Chapter 12 Non-invasive Prenatal Detection (NIPD) of Copy number variation (CNV) and sub-chromosomal variations.- Chapter 13 Circulation transcriptome of maternal plasma and its use in Non-invasive Prenatal Screening (NIPS).- Chapter 14 Challenges and ethical issues surrounding Non-invasive Prenatal Screening (NIPS).- Chapter 15 Implementation of Non-invasive Prenatal Screening (NIPS) in clinical practice: Comparison of developed versus developing countries.- Chapter 16 Sequencing: A promising path in the detection of fetal health.