NMDA Receptor Protocols

• Isolation of Receptor Clones by Expression Screening in Xenopus Oocytes.- Expression of NMDA Receptor Channel Subunit Proteins Using Baculovirus and Herpesvirus Vectors.- Transient Expression of Functional NMDA Receptors in Mammalian Cells.- Stable Expression of Human NMDA Receptors in Cultured Mammalian Cells.- Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells.- Immunocytochemistry of NMDA Receptors.- The Use of Ligand Binding in Assays of NMDA Receptor Function.- Calmodulin Modification of NMDA Receptors.- Detergent Solubilization and Immunoprecipitation of Native NMDA Receptors.- Redox Sensitivity of NMDA Receptors.- In Vitro Selection of Peptides Acting on NMDA Receptors.- NMDA Receptor Pharmacology and Analysis of Patch-Clamp Recordings.- Determination of Membrane Topology of Glutamate Receptors.- Engineering the NMDA Receptor Channel Lining.- High-Throughput Functional Detection of NMDA Receptor Activity.

"...In this volume of Methods in Molecular Biology great care has been taken to present the most modern approaches to solving the mysteries of the NMDA receptor...Each chapter includes detailed protocols and highly useful 'notes' that explain possible problems, sources of technical artifacts and how to overcome them...this book is informative and practical and would serve any channel or receptor researcher well." -Molecular Biotechnology