Nitrogen Metabolism in Plants

Methods and Protocols

Häftad, Engelska, 2020

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This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation.

An Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants.- Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues.- Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves under Different Conditions.- Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples.- Measurement of S-Nitrosoglutathione Reductase Activity in Plants.- Expression Analysis of Important Genes Involved in Nitrogen Metabolism under Hypoxia.- Enzymatic Conversions of Glutamate and γ-Aminobutyric Acid as the Indicators of Plant Stress Response.- Using Different Forms of Nitrogen to Study Hypersensitive Response Elicited by Avirulent Pseudomonas syringae.- Using Foldscope to Monitor Superoxide Production and Cell Death during Pathogen Infection in Arabidopsis under Different Nitrogen Regimes.- Methods for Estimation of Nitrogen Components in Plantsand Microorganisms.- A Precise Method for Analysing Nitrogen Use in Foxtail Millet.- Methods for Isolation and Characterization of Nitrogen Fixing Legume-Nodulating Bacteria.- Detection and Quantification of Nitrifying Bacteria using Real-Time PCR.- Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies.