Next Generation Culture Platforms for Reliable In Vitro Models

Methods and Protocols

Inbunden, Engelska, 2021

Av Tiziana A.L. Brevini, Alireza Fazeli, Kursad Turksen, Tiziana A. L. Brevini

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This detailed book collects original protocols aimed at encouraging and stimulating the scientific community to design and produce models for the laboratory that mimic cell guidance conditions as they occur in vivo.

Produktinformation

Utgivningsdatum2021-02-19
Mått178 x 254 x 23 mm
Vikt801 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor307
FörlagSpringer-Verlag New York Inc.
ISBN9781071612453

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Biokemisk teknik inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik
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Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State.- Clinostat 3D Cell Culture: Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids.- Protocol to Study the Role of Extracellular Vesicles during Induced Stem Cell Differentiation.- A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydrophobic Microbioreactors.- Use of Transparent Liquid Marble: Microbioreactor to Culture Cardiospheres.- Isolation, Culture, and Characterization of Primary Bovine Endometrial, Epithelial, and Stromal Cells for 3D In Vitro Tissue Models.- Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissues.- Use of Virus-Mimicking Nanoparticles to Investigate Early Infection Events in Upper Airway 3D Models.- Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bio-Scaffold.- A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-Like Cells.- 3D-ViaFlow: A Quantitative Viability Assay for Multicellular Spheroids.- Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometry, Comet Assay, Transcriptomics, Proteomics, and Lipidomics.- Isolation of Extracellular Vesicles (EVs) Using Size Exclusion-High Performance Liquid Chromatography (SEC-HPLC).- Isolation of Extracellular Vesicles (EVs) Using Benchtop Size Exclusion Chromatography (SEC) Columns.- Measurement of the Size and Concentration and Zeta Potential of Extracellular Vesicles Using Nanoparticle Tracking Analyzer.- Isolation, Characterization, and MicroRNAs Analysis of Extracellular Vesicles from Bovine Oviduct and Uterine Fluids.- Biomimetic 3D-Bone Tissue Model.- Using the Air-Liquid Interface Approach to Foster Apical-Basal Polarization of Mammalian FemaleReproductive Tract Epithelia In Vitro.- Preparation of Biological Scaffolds and Primary Intestinal Epithelial Cells to Efficiently 3D Model the Fish Intestinal Mucosa.- Use of Porous Polystyrene Scaffolds to Bioengineer Human Epithelial Tissues In Vitro.