Del 4 - Methods in Molecular Biology

New Nucleic Acid Techniques

Häftad, Engelska, 2013

Av John M. Walker

2 799 kr

Beställningsvara. Skickas inom 7-10 vardagar

Fri frakt för medlemmar vid köp för minst 249 kr.

Produktinformation

Utgivningsdatum2013-08-22
Mått178 x 254 x undefined mm
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor560
FörlagHumana Press Inc.
ISBN9781489943835

Tillhör följande kategorier

Biokemi inom Naturvetenskap och teknik

Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde.- Northern Blotting.- Hybrid-Release Translation.- Hybrid-Arrested Translation.- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells.- Use of Elutips to Purify DNA.- Plasmid Preparation on Sephacryl S1000.- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels.- Use of 35S Nucleotides for DNA Sequencing.- Direct Dideoxy DNA Sequencing.- Computer Applications to Molecular Biology.- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique.- Oligonucleotide Synthesis Using the Manual Phosphotriester Method.- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method.- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis.- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries.- Construction of Mammalian Genomic Libraries Using ? Replacement Vectors.- Cosmid Library Construction.- Construction of cDNA Libraries in ?gt10 or ?gt11.- Immunoscreening of ?gt11 Expression Libraries Using an Avidin–Biotin Detection System.- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides.- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors.- Expression Screening of cDNA Libraries in pEX.- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins.- Epitope Mapping Using pEX.- DNA Transfection of Mammalian Cells Using Polybrene.- Expression of Foreign Genes in Mammalian Cells.- Dot-Blot Hybridization Method.- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes.- Photobiotin-Labeled DNA and RNA Hybridization Probes.- Enzyme-Labeled DNA Probes.-Luminescent Detection of Specific DNA Sequences.- Detection of DNA Sequences Using Biotinylated Probes.- Crosslinking of Single-Stranded DNA to Resins.- Extraction of RNA from Plants.- Isolation of Plant Nuclei.- In Vitro Transcription in Plant Nuclei.- Large-Scale Isolation of Ti Plasmid DNA.- Transformation and Regeneration of Dicotyledonous Plants.- Plant Protoplast Fusion.- Plant Tissue Culture.- Direct Gene Transfer into Plant Protoplasts.- Isolation of Wheat Chloroplasts and Chloroplast DNA.- DNA Labeling by Isolated Wheat Chloroplasts.

Du kanske också är intresserad av

Lucia är död

Nyhet

Del 3

Lucia är död

Katarina Wennstam

259 kr299 kr

Död mans kvinna

Del 2

Död mans kvinna

Katarina Wennstam

79 kr125 kr

Gränsöverskridaren

Nyhet

Del 6

Gränsöverskridaren

289 kr319 kr

Kollegieblock A4 rutat - grå inlaga/vita linjer

Kollegieblock 3 för 2

Kollegieblock A4 rutat - grå inlaga/vita linjer

Kollektion Stora A

79 kr

Proteins

Del 1

Proteins

John M. Walker, John M Walker

1 409 kr

Molecular Biology and Biotechnology

Molecular Biology and Biotechnology

J. M. Walker, John M. Walker, John M. Walker, E.B. Gingold, E. B. Gingold

1 179 kr

Nucleic Acids

Del 2

Nucleic Acids

1 409 kr

Nucleic Acids

Del 2

Nucleic Acids

John M. Walker, John M Walker

1 419 kr

Protein Protocols Handbook

Protein Protocols Handbook

4 129 kr

Basic Protein and Peptide Protocols

Del 32

Basic Protein and Peptide Protocols

John M. Walker, John M Walker

2 109 kr