Neurotrophic Factors

• The Neurotrophin Family of Neurotrophic Factors – An Overview.- Neuronal Growth-Promoting and Inhibiting Cues in Neuroprotection and Neurodegeneration.- Culture of Rat Cerebellar Granule Neurons and Application to Identify Neuroprotective Agents.- Isolation and Culture of Neural Progenitor Cells from Rat Postnatal Cerebellum.- Culture of Rodent Cortical and Hippocampal Neurons.- Amyloid β-Peptide Neurotoxicity Assay Using Cultured Rat Cortical Neurons.- Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex and Spinal Cord.- Central Nervous System Neuron-Glia Co-Culture Models.- Culture and Characterization of Rat Mesencephalic Dopaminergic Neurons.- Preparation of Adult Spinal Cord Motor Neuron Cultures Under Serum-Free Conditions.- Rodent Retinal Ganglion Cell Cultures.- Culture of Purified Glial Cell Populations from Optic Nerve.- Isolation and Culture of Rat Cone Photoreceptor Cells.- Rat Retina Pigmented Epithelial Cells.- Mammalian Growth Cone Turning Assays Identify Distinct Cell Signalling Mechanisms that Underlie Axon Growth, Guidance and Regeneration.- Culture of Dissociated Sensory Neurons from Dorsal Root Ganglia of Postnatal and Adult Rats.- Culture and Proliferation of Highly Purified Adult Schwann Cells from Rat, Dog, and Man.- Use of PC12 Cells and Rat Superior Cervical Ganglion Sympathetic Neurons as Models for Neuroprotective Assays Relevant to Parkinson’s Disease.- Compartmented Chambers for Studying Neurotrophic Factor Action.- Preparation and Culture of Adrenal Chromaffin Cells.- Indirect Immunofluorescence Staining of Cultured Neural Cells.- Neurite Outgrowth Assessment Using High Content Analysis Methodology.- Dissociated Cell Culture for Testing Effects of Carbon Nanotubes on Neuronal Growth.- High-Resolution Imaging and Evaluation of Spines in Organotypic Hippocampal Slice Cultures.- Imaging Amyloid Precursor Protein In Vivo – An Axonal Transport Assay.- The Use of Specific AAV Serotypes to StablyTransduce  Primary CNS Neuron Cultures.- Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides.-  [3H]Serotonin Release Assay Using Antigen-Stimulated Rat Peritoneal Mast Cells.- Rat Hippocampal Slice Culture Models for the Evaluation of Neuroprotective Agents.- A 6-Hydroxydopamine In Vivo Model of Parkinson’s Disease.- Brain Microdialysis in Freely Moving Animals.- Evaluating Motor Neuron Death in Neonatal Rats Subjected to Sciatic Nerve Lesion.- Rodent Spinal Cord Injury Model and Application of Neurotrophic Factors for Neuroprotection.

From the reviews:“It provides a foundation for neurobiological research into the effects of neurotrophic factors and other chemicals on the nervous system. … The audience is broad, including practicing and in training neuroscientists, neurobiologists, and basic neuroscience researchers in human and veterinary medicine, as well as academic neurologists, neurosurgeons, and pharmacists. The methods can be incorporated into basic research laboratories and into medical, biology, and veterinary classes.” (Ramsis Farid Ghaly, Doody’s Book Reviews, July, 2012)