Neurophotonics and Biomedical Spectroscopy

Robert A. Alfano is Distinguished Professor and Fellow at The City College of City University of New York, USA. He is also an Optical Society of America; Fellow, and a Fellow of IEEE. He has been involved in developing ultrafast laser spectroscopic techniques and applications of these techniques to study ultrafast dynamical processes in physical, chemical, and biological systems. His research encompasses the study and development of supercontinuum, tunable solid-state lasers, nonlinear optical processes, application of optical spectroscopic techniques for medical diagnosis (optical biopsy), study of photon migration in turbid media, and development of optical imaging techniques for biomedical imaging (optical mammography). He has published more than 700 papers and holds 102 patents. Lingyan Shi is an Assistant Professor in the Department of Bioengineering at the UC San Diego Jacobs School of Engineering. She is developing and applying new ultrafast laser scanning optical imaging and spectroscopic technologies, such as stimulated Raman scattering (SRS) microscopy for solving biological questions. Her recent major scientific contributions include the discovery of the “Golden Optical Window” for deep brain imaging, and the development of a breakthrough platform that combines deuterium probing with SRS for optical imaging of metabolic dynamics in living organisms. She is combining bioorthogonal labeling and SRS imaging for directly visualizing complex molecular events in brain, tumor, and metabolic diseases at sub-cellular scale in situ, which offer powerful tools potentially for disease detection, diagnosis and treatment, as well as for mechanistic understanding of scientific fundamentals. Shi is a member of OSA, SPIE, and also a member of New York Academy of Science.

• Part 1: Topics in Optical Spectroscopy Biopsy1. Origin of tissue optical properties 2. Optical methods for tissue diagnosis and treatment3. Methods for in vivo assessment of physiological state of tissue4. Excitation, absorption, scattering fluorescence spectroscopy 5. Resonance Raman spectroscopy6. Inelastic light scattering spectroscopy and imaging7. Stimulated Raman gain spectroscopy and imaging—NIR optical windows in 700 nm to 2500 nm8. Coherent and non-contact photo-acoustic imaging9. Polarization and diffusive reflectance spectral imaging time resolved spectroscopy single and multi-photon excitation and imaging10. Mid infrared diagnostic methods11. Nano particle tagging and contrast agents optical metabolomics Demos, Gansean12. Supercontinuum for medical and biological applications13. Stokes shift spectroscopy Part 2: Topics in Optics in Brain14. Alzheimer’s Disease Optical Imaging in vascular impairment15. Hemodynamics by NIR in Brain, and Overview View summary of field16. Blood Barrier in mice using two photon and ultrasound17. Overview of Infrared neural stimulation18. 2Photon excitation in brain19. Overview NIR correlation spectroscopy in brain 20. Overview of Optical Coherent Tomography in neuron tree21. IR stimulation in spinal nerves22. Ca+2 channels in brain by multiphotons23. Ca2+ flow in brain by multiphotons24. Overview Ca2+ indicators by 2PEF25. Control Biosystems using optogentics26. Optogenetic circuit mapping with traditional whole cell patch clamp to determine synaptic connectivity between identified neurons in mouse models of psychiatric disease27. Special Proteins Red fluorescent proteins RFP in brain probing (Dr. Robert Campbell)28. OCT neural activity29. Stimulated Raman Scattering Microscopy in Brain Research30. Imaging the neurovascular coupling system with laminar optical tomography and hyperspectral two-photon microscopy31. Multi photon Imaging in neuroscience