Neuropeptides

• What Are Neuropeptides?.- Neuropeptide Localization in Non Mammalian Vertebrates.- Combined Light and Electron Microscopic Visualization of Neuropeptides and Their Receptors in Central Neurons.- Neuropeptide RNA Localization in Tissue Sections.- Intron-Specific Neuropeptide Probes.- Direct In Situ RT-PCR.- Laser Capture Microdissection and Quantitative-PCR Analysis.- Visualization of Peptide Secretory Vesicles in Living Nerve Cells.- Fluorescence Imaging with Single-Molecule Sensitivity and Fluorescence Correlation Spectroscopy (FCS) of Cell-Penetrating Neuropeptides.- Analysis of Neuro-Immune Interactions by an In Vitro Co-Culture Approach.- Electrophysiology.- Localization of Neuropeptides by Radioimmunoassay.- Reversed-Phase HPLC and Hyphenated Analytical Strategies for Peptidomics.- Neuropeptidomics: Mass Spectrometry-Based Qualitative and Quantitative Analysis.- Suppression Subtractive Hybridization.- Neuropeptide Microdialysis in Free-Moving Animals.- Antibody Microprobes for Detecting Neuropeptide Release.- Neuropeptidases.- Neuropeptide Autoantibodies Assay.- Intranasal Delivery of Neuropeptides.- Prodrug Design for Brain Delivery of Small- and Medium-Sized Neuropeptides.- Measurement of Phosphorothioate Oligodeoxynucleotide Antisense Transport Across the Blood-Brain Barrier.- Liposome-Encapsulated Neuropeptides for Site-Specific Microinjection.- Recombinant Adeno-Associated Viral Vectors.- De-Orphanizing G Protein-Coupled Receptors by a Calcium Mobilization Assay.

From the reviews: "Neuropeptides: Methods and Protocols is a cutting-edge resource for any lab working in the area of neuropeptides. The overall goal of this book is to highlight a variety of important methods in neuropeptide research and provide protocols for executing those protocols. ... this is a terrific contribution to neuropeptide research, a one-stop resource that will benefit all scientists interested in neuropeptides." (Alan Saghatelian, ChemBioChem, Vol. 13 (6), April, 2012)