Neurons: Methods and Applications for the Cell Biologist
Inbunden, Engelska, 2003
2 249 kr
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Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented.
Contains culture methodology for more than a dozen types of CNS and PNS neurons
Includes most recent and reliable techniques from expert practitioners for specific experimental applications
Addresses the latest strategies for transfecting neurons
Edited by Peter J. Hollenbeck and Dr. James R. Bamburg
Comparing the Properties of Neuronal Culture SystemsGrowing and Working with Peripheral NeuronsDissection and Culturing of Chick Ciliary Ganglion NeuronsThe Culture of Chick Forebrain NeuronsGrowing and Working with Spinal Motor NeuronsAvian Purkinje Neuronal CulturesCulturing Hippocampal and Cortical NeuronsWorking with Xenopus Spinal Neurons in Live Cell CultureCulturing Neurons from the Snail HelisomaThe Ti1 Pioneer PathwayTechniques to Dissect Cellular and Subcellular Function in Drosphila Nervous SystemPC12 Cells as a Model for Studies of Regulated Secretion in Neuronal and Endocrine Cells and more.
". . .invaluable for workers at all levels of cell biology." --NATURE
