Del 165 - Practical Approach Series

Neural Cell Culture

A Practical Approach

Häftad, Engelska, 1996

AvWilkin Cohen,James Ed Cohen,James Ed. Cohen,Cohen,Wilkin,Graham Wilkin

1 799 kr

Beställningsvara. Skickas inom 3-6 vardagar. Fri frakt för medlemmar vid köp för minst 249 kr.

The complexity and cellular heterogeneity of the vertebrate nervous system makes it difficult to study neural processes at the cellular and molecular levels. The establishment of cell culture model systems for almost every region and cell type in the nervous system has therefore been crucial in facilitating such studies and in heralding some key breakthroughs in our understanding of the underlying mechanisms. Neural Cell Culture: A Practical Approach brings together methods for preparing cultures from a diverse range of neural tissues and cells, from both the central and peripheral nervous systems of mammalian and avain species. Important features of this volume are the clearly illustrated dissection guides accompanying each chapter, methods for enriching and purifying distinct cell types, and guidance on optimizing cell viability and growth. Each chapter includes valuable step-by-step protocols, hints, and tips for success, advice, and background information.

Produktinformation

Utgivningsdatum1996-03-14
Mått156 x 235 x 16 mm
Vikt471 g
FormatHäftad
SpråkEngelska
SerieDel 165 i Practical Approach Series
Antal sidor268
FörlagIRL Press
ISBN9780199634842

Tillhör följande kategorier

Djurböcker inom Djur och natur
Zoologi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik
Neurologi och klinisk neurofysiologi inom Medicin

PART I - CNS NEURONS ; 1. Cerebellum: granule cells ; 2. Cerebellar Purkinje neurons ; 3. Nigral & straital neurons ; 4. Septal neurons ; 5. Proliferation & differentiation in retinal cell cultures ; 6. Purified embryonic motoneurones ; PART II - CNS GLIA AND NON-NEURONAL CELLS ; 7. Astrocytes ; 8. Oligodendrocyte lineage cells from neonatal rat brain ; 9. Micrglia: the tissue macrophage of the CNS ; 10. Brain endothelium ; PART III - PNS NEURONS AND GLIA ; 11. Neural crest cells: strategies to generate lineage diversifaction in vitro ; 13. Avian cilliary and lumbar sympathetic ganglion neurons ; 14. Enteric ganglion cells ; 15. Mouse olfactory epithelium ; 16. Schwann cell culture

Sixteen chapters provide detailed methodological protocols on how to culture different types of neural tissue ... These chapters have been written by experts who have extensive experience with the methods in their own laboratories; hence, they typically reflect the product of years of experience with variants of the protocols described. The book is very accessible and easy to use. It is well organized, and indexed ... Nothing teaches like experience, and at least this book will give you some ideas about the experience of others that will help to guide your progress. For the neuroscientist engaged in neural cell culture this manual may be useful as a general reference, and for anyone who has immediate need for a particular chapter, this manual could indeed prove a very helpful resource.