Nanoscale Imaging of Synapses

New Concepts and Opportunities

Inbunden, Engelska, 2014

AvU. Valentin Nägerl,Antoine Triller,U. Valentin Nagerl,Valentin Nägerl

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Synapses underlie rapid and flexible neural communication in the brain and they hold the key to understanding higher brain functions in health and disease. The proposed volume brings together leading researchers to review these exciting new techniques and their application in neurobiological research.

Produktinformation

Utgivningsdatum2014-02-05
Mått178 x 254 x 15 mm
Vikt596 g
FormatInbunden
SpråkEngelska
SerieNeuromethods
Antal sidor231
Upplaga2014
FörlagHumana Press Inc.
ISBN9781461491781

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Preface.- Nanoscale imaging of protein molecules at the postsynaptic densit.- Electron tomography for the study of synaptic ultrastructure in fixed brain sections.- Time-resolved electron microscopy: coupling optogenetics with high-pressure freezing.- Investigating AMPA receptors diffusion and nanoscale organization at synapses with high density single molecule tracking methods.- Visualising the ultra-structures and dynamics of synapses by single molecule nanoscopy.- Live-cell PALM of intracellular proteins in neurons.- Monitoring nanoscale mobility of small molecules with time-resolved fluorescence anisotropy imaging.- Imaging signalling transduction in single dendritic spines.- Imaging-based measures of synaptic tenacity.- Zooming in on the (peri)synaptic extracellular matrix.- Two-photon STED microscopy for imaging synapses and glia in acute brain slices.- Index.