Del 0 - Methods in Molecular Biology

Mycotoxin Protocols

Inbunden, Engelska, 2000

AvMary W. Trucksess,Albert E. Pohland,Mary W Trucksess,Albert E Pohland

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A panel of accomplished scientists describe their innovative, cutting-edge methods for determining the levels of mycotoxin contamination in foods and feeds. Almost half the methods presented involve molecular-based immunochemical or immunochemical/chromatographic techniques. The necessary equipment, reagents, and procedures are given in great detail for the analysis of a wide variety of mycotoxins, including aflatoxins, aflatoxin M1, cyclopiazonic acid, ochratoxin A, trichothcenes, moniliformin, fumonisins, zearlenone, Stachybotrys toxins, citrinin, patulin, ergot alkaloids, and Alternaria toxins. General techniques for mycotoxin analysis, sampling procedures for collecting representative test samples, isolation techniques, and techniques for the detection and identification of toxins and impurities are also included. Up-to-date and highly practical, Mycotoxin Protocols provides a comprehensive collection of the latest bioanalytical techniques for evaluating the contamination of mycotoxins in foods and feeds.

Produktinformation

Utgivningsdatum2000-09-06
Mått155 x 235 x 21 mm
Vikt544 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor244
Upplaga2001
FörlagHumana Press Inc.
ISBN9780896036239

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Farmakologi inom Medicin

General Techniques.- Mycotoxin Method Evaluation.- Sampling Techniques.- 3 Preparatory Isolation of Mycotoxins from Solid Phase Fungal Cultures.- 4 Preparation of Mycotoxin Standards.- Electrospray Mass Spectrometry for Mycotoxin Detection and Purity Analysis.- Methods for Aspergillus Toxins.- Measurement of Aflatoxins Using Capillary Electrophoresis.- Liquid Chromatographic Method for Aflatoxin M1 in Milk.- Immunochemical Method for Cyclopiazonic Acid.- Immunochemical Method for Ochratoxin A.- Methods for Fusarium Toxins.- Solution Fluorometric Method for Deoxynivalenol in Grains.- Chromatographic Methods forTrichothecenes.- Chromatographic Method for the Determination of the Mycotoxin Moniliformin in Corn.- Liquid Chromatographic Method for Fumonisins in Corn.- Enzyme-Linked Immunosorbent Assays of Zearalenone Using Polyclonal, Monoclonal and Recombinant Antibodies.- Methods for Other Fungal Toxins.- 15 Chromatographic Method for StachybotrysToxins.- 16 Immunochemical Method for Citrinin.- Solid Phase Extraction Method for Patulin in Apple Juice and Unfiltered Apple Juice.- Liquid Chromatographic Method for the Determination of Ergot Alkaloids in Cereal Grains.- Chromatographic Method for AlternariaToxins in Apple Juice.

"...many analysts will be attracted by this book in which assays for several mycotoxins are described." - MicroBiology Today "After reading the complete book, I would like to congratulate the editors as well as all the authors for work well-done. This book is well-written and could be used both as a practical laboratory handbook as well as a reference book for research scientists. The authors have not only described the protocols in detail, but also reviewd different methodologies and provided notes of their own experience in some of the protocols. These notes are extremely useful for the readeres to understand the rationales, difficulties, steps to overcome the problems, alternatives for certain steps, precautions for certain steps as well as approaches to modify some steps/protocols for other related mycotoxins. " - Journal of Food Biochemistry