Mycobacterium Tuberculosis Protocols

Inbunden, Engelska, 2001

Av Tanya Parish, Neil G. Stoker, Neil G Stoker

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Leading investigators with extensive practical knowledge and experience describe their best methods for studying the tuberculosis pathogen. Packed with step-by-step instructions to ensure successful results, these methods range from basic handling techniques to the application of functional genomics. Highlights include methods for the basic safety and culture of M. tuberculosis, fractionation of the bacterium (nucleic acids, lipids, culture filtrate, and capsule), the analysis of gene expression (start site mapping, real-time PCR microarrays, and proteomics), the growth of the bacterium in macrophages and low oxygen, cytological analysis of the bacteria, and diagnostics. Highly practical and accessible, Mycobacterium tuberculosis Protocols utilizes advanced functional genomics and mutagenesis methodologies to provide both experimental and clinical investigators all the powerful techniques needed to illuminate the molecular biology of tuberculosis and its interactions with host cells, and so drive work on the wide variety of emerging therapeutic opportunities.

Produktinformation

Utgivningsdatum2001-05-19
Mått155 x 235 x 31 mm
Vikt798 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Medicine
Antal sidor406
Upplaga2001
FörlagHumana Press Inc.
ISBN9780896037762

Tillhör följande kategorier

Lungmedicin inom Medicin
Infektionssjukdomar och smittsamma sjukdomar inom Medicin

Plasmid Vectors.- Isolation of DNA from Mycobacterium tubercolosis.- Extraction of RNA from Intracellular Mycobacterium tuberculosis.- Transposon Mutagenesis in Mycobacteria Using Conditionally Replicating Mycobacteriophages.- Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB.- Gene Replacement using Pretreated DNA.- Gene Replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG Using rpsL as a Dominant Negative Selectable Marker.- Transcription Start-Site Mapping.- Fluorescence and Brightfield Cytology of Live M. tuberculosis Cells.- Phage Replication Technology for Diagnosis and Drug Susceptibility Testing.- Detection of Mutations in Mycobacterium tuberculosis by a Dot Blot Hybridization Strategy.- Restriction Fragment Length Polymorphism Typing of Mycobacteria.- Preparation of Culture Filtrate Proteins from Mycobacterium tuberculosis.- Analysis of the Capsule of Mycobacterium tuberculosis.- Analysis of the Lipids of Mycobacterium tuberculosis.- In Vitro Model of Hypoxically Induced Nonreplicating Persistence of Mycobacterium tuberculosis.- 17 Macrophage Virulence Assays.- Analysis of Mycobacterium-Infected Macrophages by Immunoelectron Microscopy and Cell Fractionation.- Real Time PCR Using Molecular Beacons.- Electronic Access to Mycobacterium tuberculosis Sequence Data.- Proteomics.- Functional Genomics of Mycobacterium tuberculosis Using DNA Microarrays.- Storage of Mycobacterial Strains.- Safety in the Laboratory.

...a very useful book for the mycobacteria research community. It is a specialist's cookbook, perfect if you want to establish a new method that is outside your scope of expertise. Individual chapters are clearly divided into introduction, materials, methods and an extensive list of notes. -- International Journal of Medical Microbiology "For those engaged in mycobacterial research (and possibly other unrelated genera) which involves genetics, biochemistry and immunology, this textbook is essential and, moreover, I would recommend it as reading material for students undertaking molecular biology experimentation." -Today's Life Science