Del 732 - Methods in Enzymology

Mucins

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Inbunden, Engelska, 2026

2 769 kr

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Mucins are large, heavily glycosylated proteins that play critical roles in protecting and lubricating epithelial surfaces throughout the body, especially in the gastrointestinal, respiratory, and reproductive tracts. They are central to host-microbe interactions, immune modulation, and barrier function, and their complex structures and diverse functions make them both scientifically fascinating and technically challenging to study. Mucins serves as a valuable resource for researchers aiming to investigate mucin biology in greater depth, offering a wide range of experimental approaches and analytical strategies. It presents a comprehensive exploration of mucin biology and glycoproteomics, featuring cutting-edge methodologies and insights from leading researchers across the field. Topics span from enrichment and isolation techniques, mucinase characterization, and advanced glycoproteomic workflows to clinical applications such as lectin-based assays and mucin quantification. The chapters delve into innovative tools including CRISPR screens, cell-based mucin arrays, cryoEM, MD simulations, and microscopy, as well as synthetic approaches to mucin glycopeptides and selective sulfation. Special focus is given to mucin interactions with microbiota and pathogens, their roles in innate immunity and inflammatory bowel disease, and emerging technologies like FRET-based screening and glycan/lectin arrays. This multidisciplinary volume integrates biochemistry, microbiology, structural biology, and clinical science, serving as a foundational reference for researchers investigating mucins and their complex biological functions.

Covers a wide range of cutting-edge methodologies for studying mucins and glycoproteins
Integrates basic science with clinical and translational research applications
Features contributions from leading experts across multiple scientific disciplines

Produktinformation

Utgivningsdatum2026-07-01
Mått152 x 229 x undefined mm
FormatInbunden
SpråkEngelska
SerieMethods in Enzymology
Antal sidor258
FörlagElsevier Science
MedarbetareMalaker,Stacy
ISBN9780443432262

Tillhör följande kategorier

Tillämpad fysik inom Naturvetenskap och teknik
Biokemi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering

1. Enrichment/isolation, glycoproteomicsStacy Malaker, Shane Finn and Sarah Lowery2. Mucinase cleavage characterization and usage in glycoproteomicsStacy Malaker, Alexandra Steigmeyer and Joann Chongsaritsinsuk3. Fluorescent protein-based FRET substrates for activity screening of mucinasesAlisdair Boraston4. Development of clinical lectin assays based on MS glycomic discoveryNiclas Karlsson, Ali Reza Afshari and Mary Solbego5. Enzymatic selective sulfation of synthetic mucinsJessica Kramer6. cryoEMDeborah Fass7. Cell-based mucin arrays and glycoengineeringYoshiki Narimatsu, Christian Bull, Henrik Clausen and Felix Goerdeler8. CRISPR screens to characterize and identify glycan-binding proteinsSimon Wisnovsky9. MD SimulationsFiona Kearns10. Interactions with pathogenic bacteriaKatharina Ribbeck11. Interactions with microbiotaEric Martens12. Covalent crosslinking of mucinsThomas Crouzier13. Liquid Glycan and Liquid Lectin Array-based exploration of musin recognitionRatmir Derda14. Quantification of mucinsSumaira Z. Hasnain15. Mucins in IBDKirk Bergstrom16. Investigatins mucins and innate immunityThaher Pelaseyed17. Generating synthetic mucin glycopeptidesPeter Seeberger, Kabita Pradhan and Charlotte Völkel18. Microscopy and imagingMalin E.V. Johansson, George M H Birchenough, Elisabeth E L Nyström and Brendan Dolan