Molecular Methods for Microbial Identification and Typing

Häftad, Engelska, 1993

Av K.J. Towner, A. Cockayne, K. J. Towner

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The accurate identification and typing of microbes is essential for workers active in all fields of microbiology. Many examples of modern molecular methods have up to now been concealed in the scientific and medical literature but this introductory text considers the possible applications of such methods and compares their advantages and disadvantages. This is not a "recipe" book but is intended as a first point of reference for workers seeking an introduction to these techniques. Following the general introductory chapter, each section of the book comprises an introduction to the principles of a particular method, an overview of the methodology involved, examples of applications involving each particular method, and full references to detailed methodology and other possible applications. Methods dealt with include plasmid and chromosomal fingerprinting techniques, RFLP analysis, ribotyping, polypeptide and LPS analysis, immunoblotting, multilocus enzyme analysis and the new technique of PCR typing. This book should be of interest to clinicians, research workers and postgraduates in the field of medical microbiology.

Produktinformation

Utgivningsdatum1993-09-30
Mått155 x 235 x 12 mm
Vikt330 g
FormatHäftad
SpråkEngelska
Antal sidor208
FörlagChapman and Hall
ISBN9780412493904

Tillhör följande kategorier

1 An introduction to microbial identification and typing methods.- 1.1 A brief overview of microbial identification.- 1.2 Conventional biological methods for microbial typing.- 1.3 Molecular approaches to microbial identification and typing.- References.- 2 Analysis of nucleic acid profiles.- 2.1 Introduction.- 2.2 Plasmid analysis.- 2.3 Analysis of chromosomal DNA.- 2.4 Summary.- References.- 3 Identification and typing by nucleic acid hybridization techniques.- 3.1 Introduction.- 3.2 Basic principles of nucleic acid hybridization.- 3.3 Analysis of RFLPs by hybridization techniques.- 3.4 Summary.- References.- 4 Nucleic acid amplification and sequencing techniques.- 4.1 Introduction.- 4.2 The polymerase chain reaction (PCR).- 4.3 Other amplification methods.- 4.4 Nucleic acid sequencing techniques.- 4.5 Summary.- References.- 5 Analysis of protein and lipopolysaccharide profiles.- 5.1 Introduction to protein fingerprinting.- 5.2 Protein analysis.- 5.3 Lipopolysaccharide typing schemes.- 5.4 Summary.- References.- 6 Typing and identification of microorganisms with antibodies.- 6.1 Introduction.- 6.2 Production of antibodies for serotyping and identification.- 6.3 Techniques used in serotyping.- 6.4 Summary.- References.- 7 Final summary and future prospects.