Molecular Diagnostics

Diwakar Singh: Assistant Professor (Biochemistry), Department of Plant Molecular Biology and Biotechnology,, ASPEE College of Horticulture and Forestry,, Navsari Agricultural University, Eru Char Rasta, Dandi Road, Navsari - 396 450, Gujarat – IndiaK.P. Suthar: Assistant Professor, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Eru Char Rasta, Dandi Road, Navsari - 396 450, Gujarat – India Reetu Mehta: Lecturer, Naranlala College of Professional and applied Sciences, Navsari-396450, Gujarat, India

• Diagnosis of Drought Tolerance in Crop Plants by PEG MethodIdentification of Salt Tolerant Crop Variety by Glycine Betaine (GB) MethodTotal Protein Estimation by Folin-Lowry MethodProtein Electrophoresis Using SDS-PAGEEnzyme Extraction and Assay Study of Polyphenol Oxidase (PPO; EC 1.14.18.1)Enzyme Extraction and Assay Study of Catalase (EC 1.11.1.6)Enzyme Extraction and Assay Study of Guaiacol Peroxidase (POX) (EC 1.11.1.7)Enzyme Extraction and Activity Study of Superoxide Dismutase (EC 1.15.1.1)Peroxidase Isozyme Study by Electrophoresis MethodDNA Isolation from Plant Sample and Quantification by SpectrophotometerQuality Check of Plant Genomic DNA on Agarose Gel ElectrophoresisRandomly Amplified Polymorphic DNA (RAPD) and ElectrophoresisSouthern Blotting ExperimentTotal RNA Isolation from Plants and its Quantification by SpectrophotometerQuality Check of Total RNA on Agarose GelcDNA Synthesis from Isolated Plant Total RNAIndirect Competitive ELISAObjective Questions