Molecular Chaperones

Methods and Protocols

Häftad, Engelska, 2016

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The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form.

Produktinformation

Utgivningsdatum2016-08-23
Mått178 x 254 x 19 mm
Vikt641 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor321
FörlagHumana Press Inc.
ISBN9781493961702

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Biokemi inom Naturvetenskap och teknik

Targeted Deletion of Hsf1, 2, and 4 Genes in Mice.- The Role Of Heat Shock Factors in Stress-Induced Transcription.- Hsp90 and Client Protein Maturation.- The Role of p23, Hop, Immunophilins, and Other Co-Chaperones in Regulating Hsp90 Function.- Detecting Hsp90 Phosphorylation.- Role of Molecular Chaperones in Biogenesis of the Protein Kinome.- Nucleotide Exchange Factors for Hsp70 Chaperones.- Reconstitution of CHIP E3 Ubiquitin Ligase Activity.- Structure-Functions of HspB1 (Hsp27).- Combined Lentiviral and RNAi Technologies for the Delivery and Permanently Silencing of the hsp25 Gene.- Quantification of HSP27 and HSP70 Molecular Chaperone Activities.- Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA.- Analysis of Heat Shock Protein Localization Using Flow Cytometry.- Quantitation of Heat Shock Proteins in Clinical Samples Using Mass Spectrometry.- Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks.- Hsp70: Anti-Apoptotic and Tumorigenic Protein.- Determination of Cell Survival or Death.- Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer.- Preparation of a Heat Shock Proteins 70-Based Vaccine from DC-Tumor Fusion Cells.- Isolation of Heat Shock Protein Complexes.- Enhancing Antigen Cross-Presentation and T-Cell Priming by Complexing Protein Antigen to Recombinant Large Heat Shock Protein.- Investigating Receptors for Extracellular Heat Shock Proteins.- Analysis of Cellular Migration Using a Two-Chamber Methodology.

From the reviews:“This book details current methods and protocols essential for understanding the biological properties and roles of the molecular chaperones … . It appears to be written for all scientists interested in understanding the roles of molecular chaperones, which would include students and researchers interested in heat shock proteins. … This will be a useful guide for all scientists interested in the field of heat shock proteins and molecular chaperones and their role in health and disease.” (Omer Iqbal, Doody’s Book Reviews, December, 2011)