MicroRNAs in Development

Methods and Protocols

Häftad, Engelska, 2016

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Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants.

Produktinformation

Utgivningsdatum2016-08-23
Mått178 x 254 x undefined mm
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor200
FörlagHumana Press Inc.
ISBN9781493957941

Tillhör följande kategorier

Biovetenskap inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

In situ Detection of microRNAs in Animals.- Detection of microRNAs in Plants by In situ Hybridization.- Detecting Animal and Plant microRNAs by Northern Blotting.- Profiling microRNAs by Real-time PCR.- Detection of Small RNAs and microRNAs using Deep Sequencing Technology.- Detection of microRNAs in Prostate Cancer Cells by microRNA Array.- microRNA Knock Down by Cholesterol Conjugated Antisense Oligos in Mouse Organ Culture.- Protocols for Use of Homologous Recombination Gene-targeting to Produce microRNA Mutants in Drosophila.- Engineering Elements for Gene Silencing: The Artificial microRNAs Technology.- Mimicry Technology: Suppressing Small RNA Activity in Plants.- Experimental Validation of MicroRNA Targets using a Luciferase Reporter System.- Experimental Identification of microRNA Targets by Immunoprecipitation of Argonaute Protein Complexes.- Comprehensive Identification of miRNA Target Sites in Live Animals.- Target Validation of Plant microRNAs.- A High-throughput Sequencing-based Methodology to Identify All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes

From the reviews:“Well edited book by Prof. Tamas Dalmay provides a complete (as for today !) overview of the role played by microRNAs … . Thanks to this book I think that a multitude of readers can better understand how the genome express itself in time and space. … gives to the scientific community a clear vision of a five dimensional world in which to investigate: the three anatomical spaces (x, y and z) plus the heterochronic and heterotopic dimensions of gene expressions thanks to the microRNAs.” (Manuela Monti, European Journal of Histochemistry, July, 2011)