Microbial Ecology

Dr. Bhagwan (MSc, PhD, MCA) is an eminent researcher in Microbiome Diversity and Microbiome. After completing his postdoctoral fellowship at BRIC-NCCS (Pune), he joined Savitribai Phule Pune University, often called "the Oxford of the East," as an Assistant Professor. He is an Associate Professor at the Yenepoya Research Centre, Yenepoya (Deemed to be University) in Mangalore, India. His current specialization is Computational Biology and Evolutionary Microbiology.He serves as the expert for the European Commission, the French Foundation for Medical Research (France), the National Research and Development Agency (Chile), Certified Educator and Ambassador for GPTZero (USA), and the Academic Advisor for Fengkai Group Co., Limited (Hong Kong) for various international and national agencies. Since 2022, he has consistently been ranked on the AD Scientific Index (five times, ID: 4509536). His research is featured by Nature and The Times of India.

• Preface. Samples and Sampling Methods for the Study of Microbial Diversity from Soil and Water. Physicochemical Characterization of Soil and Water Samples. Microbiological Characterization of Soil and Water Samples. Cultivation, Isolation, and Pure Culture of Bacteria and Archaea. Short-term and Long-term Preservation of Microorganisms at Extremely Low Temperatures. Long-term Preservation of Microorganisms using Freeze-drying. Biochemical Characterization of Bacteria. Tests for Screening: Aminopeptidase, KOH Test, and Antimicrobial Resistance. Bacterial DNA Extraction. A Detailed Method of Bacterial DNA Extraction using Sonication. DNA Quantification and Agarose Gel Electrophoresis. Amplification of the 16S rRNA using Polymerase Chain Reaction (PCR). PEG-NaCl Purification of PCR Products: Principles, Protocols, and Applications. 16S rRNA Gene Sequencing Method and Bioinformatic Analysis. Software Tools for Sequence Analysis and Identification. Deposition of Sequence Data in INSDC, ENA, and DDBJ Repositories. Deposition of Pure Cultures in International Culture Collections. Primer Design for Polymerase Chain Reactions (PCRs) and Sequencing PCRs. Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Microbial Gene Detection. Multilocus Sequence Typing (MLST) for Microbial Identification and Classification. Quantitative Polymerase Chain Reaction (qPCR) Protocol and Applications. Whole Genome Sequencing (WGS) of Bacteria. Bacterial and Archaeal Genome Annotation. Denaturing Gradient Gel Electrophoresis (DGGE) Protocol and its Applications in Microbial Ecology. Index.