Micro- and Nanoengineering of the Cell Surface

Dr. Jeff Karp is a leading researcher in the fields of stem cell therapeutics, drug delivery, medical devices,and tissue adhesives. He is an Associate Professor at Brigham and Women’s Hospital, Harvard Medical School, Principal Faculty at the Harvard Stem Cell Institute, and an affiliate faculty at the Broad Institute and at the Harvard-MIT Division of Health Sciences and Technology (where he teaches to MIT-Sloan business school students). He has published >100 peer-reviewed papers (with >11,750 citations) and has given 250 national and international invited lectures and has 65 issued or pending patents. Several technologies developed in his lab have formed the foundation for multiple products on the market and currently under development and for the launch of four companies including Skintifique, Gecko Biomedical, Alivio Therapeutics, Frequency Therapeutics.Karp’s work has been discussed in hundreds of newspapers, online websites, television newscasts, and radio shows around the world including CNN, ABC News, NBC, Boston Globe, LA Times, BBC, Discovery, National Geographic, The Atlantic, The Guardian, American Museum for Natural History, Popular Mechanics, CTV Canada AM. Karp has also appeared multiple times on CBC’s Quirks and Quarks and NPR Science Fridays.The Boston Business Journal recognized him as a Champion in Healthcare Innovation and MIT’s Technology Review Magazine (TR35) also recognized Dr. Karp as being one of the top innovators in the world (3 members from his laboratory have subsequently received this award).His work has been selected by Popular Mechanic's "Top 20 New Biotech Breakthroughs that Will Change Medicine.” He gave a TEDMED talk at the Kennedy Center in DC on bioinspired medical innovation and since 2015 has been a member of the TEDMED Editorial Advisory Board (only member to be elected to the board 3 years in a row). In 2015 and 2016 he received Breakthrough Awards from the Kenneth Rainin Foundation and in 2015 was a commencement speaker in at the University of Toronto. He also serves as a consultant to the Kenneth Rainin Foundation and the Crohn’s and Colitis Foundation on the topic of new technologies for Inflammatory Bowel Disease. He has deep consulting expertise with startups and with several Fortune 500 companies across a wide spectrum of areas including drug delivery, medical devices, consumer healthcare products, and stem cell therapeutics.Dr. Karp was selected as the Outstanding Faculty Undergraduate Mentor among all Faculty at MIT and he received the HST McMahon Mentoring award for being the top mentor of Harvard-MIT students. To date, 18 trainees from his laboratory have secured faculty positions and several have transitioned into impactful careers in pharma, biotech, medtech, and venture capital. Assistant Professor, Department of Pharmaceutical Sciences, University of California, Irvine

• 1 - Cell membrane engineering: an introduction2 - Cell membrane biology3 - Cell Surface Engineering by Chemical Reaction and Remodeling4 - Noncovalent approaches5 - liposome fusion6 - fatty acid, GPI and cholesterol anchors7 - Engineering the surface of cells using the biotin-avidin interaction8 - Cell microencapsulation for tissue engineering and regenerative medicine9 - glycosylphosphatidyl inositol (GPI), cholesterol anchors, palmitated protein A/G (hydrophobic), Protein painting/transfer, tethered ligands10 - Recent advances in bioconjugation of living cells and their applications in metabolic oligosaccharide engineering.11 -bio-orthogonal chemistry at cell surfaces12 - Cell surface enzymatic engineering-based approaches to improve cell therapies13 - Strategies for incorporation of biological cues into synthetic hydrogels14 - Nanoparticle/cell interfacing, Control the stability of functioned (nano)materials on the cell surface: consideration and factors15 - Study/imaging of cell surface engineering; tools for study cell-materials interface16 - Cell surface/nanoparticle interfacing to manipulate cell fate17 - Pushing the bacterial envelope: Genetic strategies for re-engineering bacterial surfaces with heterologous proteins and sugars18 - Use of surface engineering for in vitro research tools and reagents19 - Nucleic acids as nanoscale cellular patches20 - Molecular Engineering of Cell and Tissue Surfaces with Polymer Thin Films21 - Considerations for manufacturing surface modified cells for cell therapy applications, Challenges, regulatory, scale up, future