Methods in Cyanobacterial Research

Dr. Shailendra P. Singh is an Assistant Professor at the Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University (BHU), Varanasi, India. He earned his PhD from the Department of Biology, Friedrich-Alexander University, Erlangen-Nuremberg, Germany.Prof. Dr. Rajeshwar P. Sinha, DAAD Fellowship Awardee and Fellow, Society for Applied Biotechnology, India, is a Professor of Molecular Biology, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University (BHU), Varanasi, India.Prof. Donat-P. Häder is the past-Director of the Botanical Institute and held the chair of Ecophysiology of Plants at the Friedrich-Alexander University, Erlangen-Nürnberg, Germany. He was also the director of the Botanical Garden.

• Chapter 1: Evaluating the polyphasic approach in cyanobacterial taxonomyChapter 2: Estimation of growth and photosynthetic pigments in cyanobacteriaChapter 3: Quantitative estimation of total carbohydrate, protein and lipid contents in cyanobacteriaChapter 4: Isolation and purification of phycobiliproteinsChapter 5: Methods for estimation of oxidative stress indices in cyanobacteriaChapter 6: Estimation of glutamine synthetase enzymeChapter 7: Estimation of nitrate reductase enzymeChapter 8: Estimation of nitrogenase enzyme activity in cyanobacteriaChapter 9: Isolation and purification of cyanobacterial toxinsChapter 10: Isolation and purification of scytoneminChapter 11: Isolation and purification of mycosporine-like amino acidsChapter 12: Protein estimation in cyanobacterial samplesChapter 13: Proteomic analysis of cyanobacteria employing 2-dimensional gel electrophoresis (2-DE)Chapter 14: LC-MS/MS based proteomics study of cyanobacteriaChapter 15: Monitoring photosynthetic performance in cyanobacteria by a modulated fluorometerChapter 16: Reactive oxygen species measurement in cyanobacteria using 2′,7′- dichlorodihydrofluorescein diacetate (DCFH-DA) dye and fluorescence microscope or flow cytometerChapter 17: Flow cytometry-based methods for estimating DNA content and live-dead cells in cyanobacteriaChapter 18: Genomic DNA and RNA extraction from cyanobacteriaChapter 19: Detection and quantification of DNA damage in cyanobacteria by immunodot- blot assayChapter 20: Engineering cyanobacteria for the synthesis of novel productsChapter 21: Sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteriaChapter 22: Cyanobacterial biofertilizer production technologyChapter 23: Biomass composition measurement and intracellular flux analyses for insights into cyanobacterial metabolismChapter 24: In silico genome assembly and functional annotationIndex