Methods in Cilia and Flagella

Wallace Marshall is an electrical engineer by training, who became interested in biology out of a desire to understand how cells solve engineering problems, such as determining the size of organelles. He received his Ph.D. at UCSF with John Sedat, where he studied the diffusional of motion of interphase chromatin using live cell imaging and computational image analysis. He then trained as a postdoc with Joel Rosenbaum at Yale, where he began studying the mechanisms regulating the length of cilia and flagella. He is now Profess of Biochemistry at UCSF, where he lab continues to study the assembly and length regulation of cilia and flagella, as well as the mechanisms that regulate the size of other organelles. His work takes advantage of an integrated combination of methods including genetics, microscopy, and computational modeling, as well as a wide variety of model organisms including Chlamydomonas reinhardtii, Stentor coeruleus, yeast, flatworms, and mammalian cells.

• 1. Imaging centrosomes and cilia in the mouse kidneyMasato Hoshi, Jinzhi Wang, Sanjay Jain and Moe R. Mahjoub2. Ependymal cell differentiation, from monociliated to multiciliated cellsNathalie Delgehyr, Alice Meunier, Marion Faucourt, Montserrat Bosch Grau, Laetitia Strehl, Carsten Janke, Nathalie Spassky3. Observing planar cell polarity in multiciliated mouse airway epithelial cellsEszter K. Vladar, Yin Loon Lee, Tim Stearns and Jeffrey D. Axelrod4. Imaging and analysing primary cilia in cardiac cellsNicolas Diguet, Jean-François Le Garrec, Tommaso Lucchesi and Sigolène M. Meilhac5. Cilia in photoreceptorsLinjing Li, Manisha Anand, Kollu Nageswara Rao and Hemant Khanna6. Analysis of primary cilia in the developing mouse brainJudith TML Paridaen, Wieland B Huttner and Michaela Wilsch-Bräuninger7. In vivo investigation of cilia structure and function using XenopusEric R. Brooks and John B. Wallingford8. A quantitative approach to study endothelial cilia bending stiffness during blood flow mechanodetection in-vivoFrancesco Boselli, Jacky G. Goetz, Gilles Charvin, Julien Vermot9. Quantitative description of fluid flows produced by left-right cilia in zebrafishCraig Fox, M. Lisa Manning and Jeffrey D. Amack10. Efficient live fluorescence imaging of intraflagellar transport in mammalian primary ciliaHiroaki Ishikawa and Wallace F. Marshall11. Analysis of soluble protein entry into primary cilia using semi-permeabilized cellsDavid K. Breslow and Maxence V. Nachury12. Methods for imaging individual cilia in living echinoid embryosRobert L. Morris, Hans W. Pope, Adam N. Sholi, Leah M. Williams, Chelsea R. Ettinger, Gwendolyn M. Beacham, Tatsushi Shintaku, Zachary D. Abbott, Elyse M. Doherty13. The planarian Schmidtea mediterranea as a model for studying motile cilia and multiciliated cellsCyril Basquin, Anne-Marie Orfila and Juliette Azimzadeh14. Quantitative analysis of flagellar proteins in Drosophila sperm tailsTeresa Mendes Maia, Perrine Paul-Gilloteaux, Renata Basto15. Imaging cilia in Drosophila melanogasterJennifer Vieillard, Jean-Luc Duteyrat, Elisabeth Cortier, Bénédicte Durand 16. Studying G-protein-coupled receptors: Immunoblotting, immunoprecipitation, phosphorylation, surface labeling, and cross-linking protocolsKasturi Pal, Hemant Badgandi, and Saikat Mukhopadhyay17. Image analysis of Caenorhabditis elegans ciliary transition zone structure, ultrastructure, molecular composition and functionAnna A. W. M. Sanders, Julie Kennedy and Oliver E. Blacque18. Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtiiHuawen Lin and Susan K. Dutcher19. Axonemal motility in ChlamydomonasKen-ichi Wakabayashi and Ritsu Kamiya