Methods for the Study of Microbial Biofilms

Dr. Abhishek Guldhe is currently working as Associate Professor, Amity institute of Biotechnology, Amity University Maharashtra, Mumbai. He was awarded with prestigious Ramalingaswami Re-entry Fellowship of Department of Biotechnology, Government of India in 2019. He has featured in the list of “World’s top 2% scientists” published by Stanford University, USA. His research focus area is microalgal biotechnology, bioactive compounds, and waste beneficiation. He has worked in many national and multinational funded projects with local governing bodies, industry, and international research groups. He has presented his work in international conferences and workshops in India, South Africa, Brazil, USA, and Canada. Dr. Vinothkannan completed his graduation from SASTRA University, Thanjavur, Tamil Nadu, India. He began his research career as a Research Associate at IIT Madras, followed by a postdoctoral fellowship at the Shandong University-Helmholtz Institute of Biotechnology, China. His primary research focuses on drug design targeting quorum sensing pathways in multidrug-resistant bacterial pathogens. He is currently a faculty member at Amity University, Mumbai, where he teaches courses in biochemistry and bioinformatics. Dr. Vinothkannan has authored 56 publications in peer-reviewed journals and is a recipient of several prestigious awards, including the China Postdoctoral Science Foundation Grant, the Shandong University International Exchange Fellowship, the DST-SERB Start-up Research Grant, and the DST NIDHI PRAYAS Grant. In addition to his research and teaching contributions, he serves as an Academic Editor for PLOS ONE and PLOS Pathogens and is also a member of the editorial board of Scientific Reports. Dr. Shashank Kamble holds the expertise in the biology of infectious diseases with an emphasis on tuberculosis & a consistent academic background in microbiology. He holds a PhD from the CSIR-Institute of Genomics & Integrative Biology, New Delhi and a post-doctoral training from the Jawaharlal Nehru University. Currently, he is serving as the Assistant Professor at the Amity Institute of Biotechnology, Mumbai and a visiting scientist at the Advanced Centre for Treatment, Research and Education in Cancer, Mumbai funded by the Dept. of Biotechnology, Government of India. He has several peer reviewed international publications & has delivered invited talks in various academic institutions across the country. He has received research grants from national funding agencies. Currently, He is serving as manuscript reviewer for many international peer reviewed journals such as Frontiers in microbiology, BMC microbiology, Archives of microbiology etc. His research interests include the microbial adaptations inside the hosts as well as the mechanism of anti-microbial resistance & combating strategies.

• 1. Microbial biofilms: Occurrence and implications2. In silico methods to study biofilms3. In vitro methods to study biofilms4. Microscopic methods for biofilm analysis5. In vivo models for biofilms6. Genomic approaches to study biofilms7. Transcriptomics approaches: qPCR-based techniques8. Transcriptomics approaches: Microarray and RNA sequencing-based techniques9. Proteomics studies of biofilms10. Metabolomics studies11. Metagenomics/population studies for biofilms12. Proteogenomic, a latest tool for simultaneous identification of microbial dynamics and proteins involved in biofilm formation13. Mathematical tools to understand biofilms

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