Methods for the Discovery and Characterization of G Protein-Coupled Receptors

• In Silico Identification of Novel G Protein-Coupled Receptors.- Alternative Pre-mRNA Splicing of G Protein-Coupled Receptors.- Detecting Polymorphisms in G Protein-Coupled Receptor Genes.- Regulation of the Transcription of G Protein-Coupled Receptor Genes.- Deciphering the Evolution of G Protein-Coupled Receptors in Vertebrates.- A New Approach to Visualize Endogenously Expressed G Protein-Coupled Receptors in Tissues and Living Cells.- Post-Translational Regulation of G Protein-Coupled Receptors.- Discovering G Protein-Coupled Receptor Motifs Mediating Export from the Endoplasmic Reticulum.- Identifying G Protein-Coupled Receptor Escorts, Chaperones, and Intracellular Tethers Regulating Receptor Density at the Cell Surface.- A Novel Method for Determining the Kinetics of G Protein-Coupled Receptor Plasma Membrane Expression.- Characterizing the Pharmacology of G Protein-Coupled Receptors in Transfected Cell Lines.- Novel Assay Technologies for the Discovery of G Protein-Coupled Receptor Drugs.- Discovering Cell-Type-Specific Patterns of G Protein-Coupled Receptor Phosphorylation.- Quantifying Allosteric Modulation of G Protein-Coupled Receptors.- Experimental Designs for the Study of Receptor-Receptor Interactions.- Elucidating Agonist-Selective Mechanisms of G Protein-Coupled Receptor Desensitization.- Detecting the Role of Arrestins in G-Protein Coupled Receptor Regulation.- Characterizing Molecular Mobility and Membrane Interactions of G Protein-Coupled Receptors.- Using RNA Interference to Downregulate G Protein-Coupled Receptors.- Upregulating G Protein-Coupled Receptors with Receptor Antagonists.

From the reviews:“Methods for the Discovery and Characterization of G Protein-Coupled Receptors is an excellent reference text for anyone concerned with the dynamic life of GPCRs in living tissues. The book is suitably organized to cater to a wide audience, and the material is presented in a logical framework to permit understanding of major concepts by a novice. … This would be a particularly valuable book for any structural biologist looking to build complexity into their experimental system to account for in vivo phenomena.” (Michelle O’Malley, ChemBioChem, Vol. 13 (6), April, 2012)