Metagenomics

Methods and Protocols

Inbunden, Engelska, 2022

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Authoritative and practical, Metagenomics: Methods and Protocols, Third Edition provides a comprehensive collection of up-to-date metagenome protocols and tools for the recovery of many major types of biocatalysts and allows for the easy setup of these screens in microbiology laboratories.

Produktinformation

Utgivningsdatum2022-10-29
Mått178 x 254 x 22 mm
Vikt765 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor287
Upplaga3
FörlagSpringer-Verlag New York Inc.
ISBN9781071627945

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

Construction of Small-Insert and Large-Insert Metagenomic Libraries.- Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies.- Functional Metagenomics as a Tool to Tap into Natural Diversity of Valuable Biotechnological Compounds.- Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA.- Identification of PKS Gene Clusters from Metagenomic Libraries Using a Next Generation Sequencing Approach.- Activity-Based Screening of Metagenomic Fosmid Libraries for Hydrogen-Uptake Enzymes.- Functional Metagenomics Approach for the Discovery of Novel Genes Encoding Phosphatase Activity.- Isolation of Genes Encoding Carbon Metabolism Pathways from Complex Microbial Communities.- Screening Metagenomes for Algae Cell Wall Carbohydrates Degrading Hydrolases in Enrichment Cultures.- The PET-Degrading Potential of Global Metagenomes: From InSilico Mining to Active Enzymes.- High-Throughput Screening for Thermostable Polyester Hydrolases.- Metagenomic Screening of a Novel PET Esterase via In Vitro Expression System.- Assigning Functions of Unknown Enzymes by High Throughput Enzyme Characterization.- Isolation of a Host-Confined Phage Metagenome Allows the Detection of Phages Both Capable and Incapable of Plaque Formation.- CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis.- Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production.- DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms.