Metabolic Profiling

This second edition volume expandson the previous edition with new chapters and updated discussions on the latest advancements in the development and practice of metabolicphenotyping. The chapters in this book cover topics such as quality control in untargeted metabolic phenotyping; implementation of quality assurances processes; careful bio- and chemoinformatic data analysis; using reversed-phase and ion-pair LC-MS; quantitative lipid analysis using supercritical fluid chromatography (SFC-MS)and thetargeted determination of metanephrines in urine via LC-MS/MS. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshootingand avoiding known pitfalls. Thorough and comprehensive,Metabolic Profiling: Methods and Protocols, Second Editionis a valuable resource for all researchers who are interested in incorporating best practices in metabolic phenotyping and learning more about thisrapidlyadvancing field.