Del i serien Springer Protocols Handbooks

Medical BioMethods Handbook

Häftad, Engelska, 2005

2 869 kr

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The numerous advances in our ability to investigate and define cellular processes at the molecular level, have been the result of the development and application of powerful new techniques in molecular biology, in particular nucleic acid and protein methodologies. The purpose of Medical Biomethods Handbook is to introduce the reader to a wide-ranging selection of those molecular biology techniques that are most frequently used by research workers in the field of medical and clinical research.

Produktinformation

Utgivningsdatum2005-03-18
Mått178 x 254 x 41 mm
Vikt1 379 g
FormatHäftad
SpråkEngelska
SerieSpringer Protocols Handbooks
Antal sidor644
Upplaga2005
FörlagHumana Press Inc.
ISBN9781588293343

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Patologi inom Medicin

Basic Techniques in Molecular Biology.- Probe Design, Production, and Applications.- Restriction Enzymes.- Southern Blotting as a Diagnostic Method.- Western Blotting as a Diagnostic Method.- Principles and Medical Applications of the Polymerase Chain Reaction.- Single-Strand Conformation Polymorphism (SSCP) Analysis.- Denaturing Gradient Gel Electrophoresis (DGGE).- Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing.- to Capillary Electrophoresis of DNA.- Mapping Techniques.- Dideoxyfingerprinting for Mutation Detection.- Conformation-Sensitive Gel Electrophoresis.- Amplification Refractory Mutation System and Molecular Diagnostics.- Ligase Chain Reaction.- Chemical Cleavage of Mismatch.- The Protein Truncation Test.- Linkage, Allele Sharing, and Association.- Single-Nucleotide Polymorphisms.- cDNA Microarrays.- Nucleic Acid Sequence-Based Amplification.- Oligonucleotide Ligation Assay.- Quantitative TaqMan Real-Time PCR.- Use of Denaturing High-Performance Liquid Chromatography in Molecular Medicine.- Quantitative PCR.- Liquid Chromatography-Mass Spectrometry of Nucleic Acids.- Comparative Genomic Hybridization in Clinical and Medical Research.- Bioinformatic Tools for Gene and Protein Sequence Analysis.- In SituHybridization.- Enzyme-Linked Immunosorbent Assay.- Protein Therapeutics.- Karyotyping.- Microsatellite Analysis.- Analysis of Chromosomal Translocations.- Differential Display.- Techniques for Gene Expression Profiling.- Capillary Electrophoresis in Clinical Analysis.- Flow Cytometry in the Biomedical Arena.- Immunocytochemistry.- Ribotyping in Clinical Microbiology.- Diagnostic Applications of Protein Microarrays.- The Human Genome Project.- Prenatal Diagnosis of Inborn Errors of Metabolism.- Gene Therapy.