Uranium hexafluoride plays a crucial role in the nuclear fuel cycle, particularly due to its use in the most widely adopted enrichment methods. This publication provides a comprehensive overview of the properties of uranium hexafluoride and the potential challenges associated with its production, transport, handling and storage. It is designed to support professionals involved in any stage of the uranium hexafluoride lifecycle by offering detailed, practical information. It is especially useful for those seeking to enhance aspects of their existing programmes, including management systems, safety protocols, emergency preparedness and response.