Manual of Practical Medical Biochemistry

Evangeline Jones MD Formerly Professor and Head, Department of Biochemistry, Vinayaka Missions Kirupanandha, Variyar Medical College and Government Mohan Kumaramangalam Medical College Salem, Tamil Nadu, India

• 1. GeneralGeneral InstructionsLaboratory Equipments and ProceduresBuffers and pHQuality Control2. Analysis of Urine and Cerebrospinal FluidNormal Urine: Characteristics and AnalysisAbnormal Constituents of UrineScreening of Urine for Inborn Errors of Metabolism and the Use of Paper ChromatographyComposition of Cerebrospinal Fluid3. Quantitative ExperimentsPrinciples of ColorimetryPrinciples of SpectrophotometerEstimation of GlucoseGlucose Tolerance TestEstimation of Serum and Urine Creatinine: Creatinine ClearanceEstimation of UreaSerum Proteins—Albumin: Globulin RatioSerum Total CholesterolLipid ProfileSerum Calcium and PhosphorusSerum BilirubinSerum TransaminasesSerum Alkaline PhosphataseSerum Uric Acid4. Equipments And ProceduresPoint of Care TestingpH MeterPaper Chromatography and Thin Layer ChromatographyProtein ElectrophoresisPolyacrylamide Gel ElectrophoresisElectrolyte AnalysisArterial Blood Gas AnalyzerEnzyme-Linked Immunosorbent AssayImmunodiffusionAutoanalyzerDNA Isolation from Blood/Tissues5. Function TestsGastric Function Tests and Analysis of Gastric JuicePancreatic Function TestsLiver Function TestsAnalysis of BileRenal Function TestsThyroid Function TestsAdrenal Function Tests6. Food and EnergyEnergy Content of Food and Glycemic IndexFats in Food7. Clinical Case StudiesBasis and Rationale of Biochemical Tests Done in Certain Clinical Conditions8. Objective Structured Practical ExaminationObjective Structured Practical Examination9. ReagentsReagent Preparation10. Normal ValuesNormal ValuesWorksheet