Del i serien Methods in Molecular Biology

Mammalian Synthetic Promoters

Inbunden, Engelska, 2017

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This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application.

Produktinformation

Utgivningsdatum2017-08-12
Mått178 x 254 x 22 mm
Vikt751 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor277
Upplaga17001
FörlagHumana Press Inc.
ISBN9781493972210

Tillhör följande kategorier

Biovetenskap inom Naturvetenskap och teknik

Initial Considerations before Designing a Promoter Construct.- Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay.- Chromatin Immunoprecipitation and Quantitative Real-time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites within a Promoter.- Secreted Reporters for Monitoring Multiple Promoter Function.- Bioluminescence Monitoring Of Promoter Activity In Vitro and In Vivo.- Monitoring Promoter Activity by Flow Cytometry.- Functional Screening of Core Promoter Activity.- Bioinformatically Informed Design of Synthetic Mammalian Promoters.- Synthetic Tumor Specific Promoters for Transcriptional Regulation of Viral Replication.- Constructing Strong Cell-Type-Specific Promoters through Informed Design.- PCR Assembly of Synthetic Promoters.- The Tetracycline Responsive System.- Light-responsive Promoters.- A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation.- Promoter Activation with Electromagnetism.- Application of Synthetic Tumor Specific Promoters Responsive to the Tumor Microenvironment.- A Modular Approach to Building Complex Synthetic Circuits.- Computational Sequence Design with R2oDNA Designer.- Design of Synthetic Promoters for Gene Circuits in Mammalian Cells.