Mark Sanderson is an experienced protein and nucleic-acid crystallographer and co-editor of Methods in Molecular Biology, who has solved several key ab initio structures, notably nucleic-acid proteins.Jane Skelly has widespread experience in the expression and crystallisation of macromolecules.Both editors lecture undergraduates in structural biology.

• 1. Classical Cloning, Expression and Purification ; 2. High-Throughput Cloning, Expression and Purification ; 3. Automation of Non-Conventional Crystallization Techniques for Screening and Optimisation ; 4. First Analysis of Macromolecular Crystals ; 5. In-House Macromolecular Data Collection ; 6. Solving the Phase Problem Using Isomorphous Replacement ; 7. Molecular Replacement Techniques for High-Throughput Structure Determination ; 8. MAD Phasing ; 9. Application of Direct Methods to Macromolecular Structure Solution ; 10. Phase Refinement through Density Modification ; 11. Getting a Macromolecular Model: Model Building, Refinement and Validation ; 12. High-Throughput Crystallographic Data Collection at Synchrotrons ; 13. Electron Density Fitting and Structure Validation ; 14. RNA Crystallogenesis ; 15. Crystallography in the Study of Protein-DNA Interaction ; 16. Virus Crystallography ; 17. Macromolecular Crystallography in Drug Design

it is more than successful, and most, if not all, who dip into it will be rewarded by learning things they did not know, or possibly had forgotten...[..] a book that will surely find its place in every crystallographic lab whether protein or macromolecular.