Long Non-Coding RNAs

Methods and Protocols

Häftad, Engelska, 2018

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This volume assembles a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA.

Produktinformation

Utgivningsdatum2018-03-31
Mått178 x 254 x 17 mm
Vikt590 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor298
FörlagHumana Press Inc.
ISBN9781493980352

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LncRNA Pulldown Combined with Mass Spectrometry to Identify the Novel LncRNA-Associated Proteins.- Cross-Linking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions between Long Non-Coding RNAs and RNA-Binding Proteins.- Characterization of Long Non-Coding RNA Associated Proteins by RNA-Immunoprecipitation.- Isolation of Protein Complexes Associated with Long Non-Coding RNAs.- Profiling Long Non-Coding RNA Expression Using Custom-Designed Microarray.- Long Non-Coding RNA Expression Profiling Using Arraystar LncRNA Microarrays.- Nuclear RNA Isolation and Sequencing.- Targeted LncRNA Sequencing with the SeqCap RNA Enrichment System.- ChIP-Seq: Library Preparation and Sequencing.- Stellaris ® RNA Fluorescence In Situ Hybridization for the Simultaneous detection of Immature and Mature Long Non-Coding RNAs in Adherent Cells.- Simultaneous RNA-DNA FISH.- Visualizing Long Non-Coding RNAs on Chromatin.- Non-Isotopic Method for In Situ LncRNA Visualization and Quantitation.- Detection of Long Non-Coding RNA Expression by Non-Radioactive Northern Blots.- Assessment of In Vitro SiRNA Delivery in Cancer Mouse Models.- Targeting Long Non-Coding RNA with Antisense Oligonucleotide Technology as Cancer Therapeutics.- Characterization of Circular RNAs.- Methods for Characterization of Alternative RNA Splicing.- NONCODEv4: Annotation of Non-Coding RNAs with Emphasis on Long Non-Coding RNAs.- Computational Analysis of LncRNA from cDNA Sequences.- AnalyzingMiRNA: LncRNA Interactions.- Long Non-Coding RNAs: An Overview.