Long Non-Coding RNAs

Methods and Protocols

Häftad, Engelska, 2022

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This second edition provides a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA.

Produktinformation

Utgivningsdatum2022-08-22
Mått178 x 254 x undefined mm
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor308
Upplaga2
FörlagSpringer-Verlag New York Inc.
ISBN9781071616994

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LncRNA pulldown Combined with Mass Spectrometry to Identify the Novel lncRNA-associated Proteins.- Cross-Linking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions of Long Noncoding RNAs with Canonical and Non-canonical RNA-binding Proteins.- Characterization of Long Non-coding RNA Associated Proteins by RNA-immunoprecipitation.- Isolation of Protein Complexes Associated with Long Non-Coding RNAs.- Detection of Long Noncoding RNA Expression by Real-time PCR.- Profiling Long Non-coding RNA expression using Custom-Designed Microarray.- Long Non-coding RNA Expression Profiling using Arraystar LncRNA Microarrays.- Nuclear RNA Isolation and Sequencing.- Capturing Endogenous Long Noncoding RNAs and Their Binding Proteins Using Chromatin Isolation by RNA Purification.- Purification and Structural Characterization of the Long Noncoding RNAs.- Simultaneous RNA-DNA FISH.- Highly Resolved Detection of Long Non-coding RNAsIn Situ.- Detection of Long Non-coding RNA Expression by Non-radioactive Northern Blots.- Assessment of in vivo siRNA Delivery in Cancer Mouse Models.- Genetic Editing of Long Non-Coding RNA Using CRISPR/Cas9 Technology.- Characterization of Circular RNAs.- Identification of circRNA-Interacting Proteins by Affinity Pulldown.- Exploration to Circular RNA Interactomes by RNA Pull-Down Method.- Methods for Characterization of Alternative RNA Splicing.- Computational analysis of lncRNA from cDNA sequences.- Characterizing miRNA-lncRNA interplay.- Illuminating lncRNA Function through Target Prediction.- Long Noncoding RNAs: An Overview.