Maarten Merkx is at Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands

• PrefaceMaarten Merkx1.Understanding and applications of Ser/Gly linkers in protein engineeringEric Ceballos-Alcantarilla and Maarten Merkx2.Design and construction of chimeric linker linkers with controllable flexibilities for precision protein engineeringZiliang Huang, Chong Zhang and Xin-Hui Xing3.Engineering crowding sensitivity into protein linkersTheodoros Pittas, Weiyan Zuo and Arnold J. Boersma4.protaTETHER: A method for the incorporation of linkers in biomacromoleculesAnna I. Wurz, Collin T. O'Bryant and Robert M. Hughes5.Optimizing the linker length for fusing an alcohol dehydrogenase with a cyclohexanone monooxygenaseAlejandro Gran-Scheuch, Friso Aalbers, Yannick Woudstra, Loreto Parra and Marco W. Fraaije6.Predicting the effect of disordered linkers on effective concentrations and avidity with the “Ceff calculator” appMagnus Kjaergaard, Juliana Glavina and Lucia Beatriz Chemes7.ER/K-link—Leveraging a native protein linker to probe dynamic cellular interactionsTejas M. Gupte, Michael Ritt and Sivaraj Sivaramakrishnan8.Design of helical linkers for fusion proteins and protein-based nanostructuresRyoichi Arai9.Synthetic protein switches: Combinatorial linker engineering with iFLinkCAlexander Gräwe, Jan Ranglack, Anastasia Weyrich and Viktor Stein10.Enzyme-DNA chimeras: Construction, allostery, applicationsChiao-Yu Tseng, Yong Wang and Giovanni Zocchi11.The Spy that links: Creation of nonlinear protein architectures and materials using SpyTag/SpyCatcher chemistryRi Wang and Fei Sun

Maarten Merkx is at Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands

• PrefaceMaarten Merkx1.Understanding and applications of Ser/Gly linkers in protein engineeringEric Ceballos-Alcantarilla and Maarten Merkx2.Design and construction of chimeric linker linkers with controllable flexibilities for precision protein engineeringZiliang Huang, Chong Zhang and Xin-Hui Xing3.Engineering crowding sensitivity into protein linkersTheodoros Pittas, Weiyan Zuo and Arnold J. Boersma4.protaTETHER: A method for the incorporation of linkers in biomacromoleculesAnna I. Wurz, Collin T. O'Bryant and Robert M. Hughes5.Optimizing the linker length for fusing an alcohol dehydrogenase with a cyclohexanone monooxygenaseAlejandro Gran-Scheuch, Friso Aalbers, Yannick Woudstra, Loreto Parra and Marco W. Fraaije6.Predicting the effect of disordered linkers on effective concentrations and avidity with the “Ceff calculator” appMagnus Kjaergaard, Juliana Glavina and Lucia Beatriz Chemes7.ER/K-link—Leveraging a native protein linker to probe dynamic cellular interactionsTejas M. Gupte, Michael Ritt and Sivaraj Sivaramakrishnan8.Design of helical linkers for fusion proteins and protein-based nanostructuresRyoichi Arai9.Synthetic protein switches: Combinatorial linker engineering with iFLinkCAlexander Gräwe, Jan Ranglack, Anastasia Weyrich and Viktor Stein10.Enzyme-DNA chimeras: Construction, allostery, applicationsChiao-Yu Tseng, Yong Wang and Giovanni Zocchi11.The Spy that links: Creation of nonlinear protein architectures and materials using SpyTag/SpyCatcher chemistryRi Wang and Fei Sun

Maarten Merkx is at Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands

• PrefaceMaarten Merkx1.Understanding and applications of Ser/Gly linkers in protein engineeringEric Ceballos-Alcantarilla and Maarten Merkx2.Design and construction of chimeric linker linkers with controllable flexibilities for precision protein engineeringZiliang Huang, Chong Zhang and Xin-Hui Xing3.Engineering crowding sensitivity into protein linkersTheodoros Pittas, Weiyan Zuo and Arnold J. Boersma4.protaTETHER: A method for the incorporation of linkers in biomacromoleculesAnna I. Wurz, Collin T. O'Bryant and Robert M. Hughes5.Optimizing the linker length for fusing an alcohol dehydrogenase with a cyclohexanone monooxygenaseAlejandro Gran-Scheuch, Friso Aalbers, Yannick Woudstra, Loreto Parra and Marco W. Fraaije6.Predicting the effect of disordered linkers on effective concentrations and avidity with the “Ceff calculator” appMagnus Kjaergaard, Juliana Glavina and Lucia Beatriz Chemes7.ER/K-link—Leveraging a native protein linker to probe dynamic cellular interactionsTejas M. Gupte, Michael Ritt and Sivaraj Sivaramakrishnan8.Design of helical linkers for fusion proteins and protein-based nanostructuresRyoichi Arai9.Synthetic protein switches: Combinatorial linker engineering with iFLinkCAlexander Gräwe, Jan Ranglack, Anastasia Weyrich and Viktor Stein10.Enzyme-DNA chimeras: Construction, allostery, applicationsChiao-Yu Tseng, Yong Wang and Giovanni Zocchi11.The Spy that links: Creation of nonlinear protein architectures and materials using SpyTag/SpyCatcher chemistryRi Wang and Fei Sun