Del 647 - Methods in Enzymology

Linkers in Biomacromolecules

Inbunden, Engelska, 2021

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Linkers in Biomacromolecules, Volume 647 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this updated release include chapters devoted to ser-gly linkers + models, sergGly- EAAAK linkers, Effect of crowding on linker behavior, Protathether. Experimental approach to quickly test different linkers in fusion proteins, Linkers in enzyme fusions/cascade catalysis, Linker dependence of avidity in multivalent interactions between disordered proteins, ER/K ?-helix linker, The effect of chirality and steric hindrance on intrinsic backbone conformational propensities: tools for protein design, Linker design in protein-based nanostructures, and much more. Linkers in Biomacromolecules, Volume 647 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this updated release include chapters devoted to ser-gly linkers + models, sergGly- EAAAK linkers, Effect of crowding on linker behavior, Protathether. Experimental approach to quickly test different linkers in fusion proteins, Linkers in enzyme fusions/cascade catalysis, Linker dependence of avidity in multivalent interactions between disordered proteins, ER/K ?-helix linker, The effect of chirality and steric hindrance on intrinsic backbone conformational propensities: tools for protein design, Linker design in protein-based nanostructures, and much more.

Provides the authority and expertise of leading contributors from an international board of authors
Presents the latest release in the Methods in Enzymology series

Produktinformation

Utgivningsdatum2021-01-21
Mått152 x 229 x undefined mm
Vikt620 g
FormatInbunden
SpråkEngelska
SerieMethods in Enzymology
Antal sidor318
FörlagElsevier Science
MedarbetareMerkx,Maarten
ISBN9780128208182

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Biokemi inom Naturvetenskap och teknik

PrefaceMaarten Merkx1.Understanding and applications of Ser/Gly linkers in protein engineeringEric Ceballos-Alcantarilla and Maarten Merkx2.Design and construction of chimeric linker linkers with controllable flexibilities for precision protein engineeringZiliang Huang, Chong Zhang and Xin-Hui Xing3.Engineering crowding sensitivity into protein linkersTheodoros Pittas, Weiyan Zuo and Arnold J. Boersma4.protaTETHER: A method for the incorporation of linkers in biomacromoleculesAnna I. Wurz, Collin T. O'Bryant and Robert M. Hughes5.Optimizing the linker length for fusing an alcohol dehydrogenase with a cyclohexanone monooxygenaseAlejandro Gran-Scheuch, Friso Aalbers, Yannick Woudstra, Loreto Parra and Marco W. Fraaije6.Predicting the effect of disordered linkers on effective concentrations and avidity with the “Ceff calculator appMagnus Kjaergaard, Juliana Glavina and Lucia Beatriz Chemes7.ER/K-link—Leveraging a native protein linker to probe dynamic cellular interactionsTejas M. Gupte, Michael Ritt and Sivaraj Sivaramakrishnan8.Design of helical linkers for fusion proteins and protein-based nanostructuresRyoichi Arai9.Synthetic protein switches: Combinatorial linker engineering with iFLinkCAlexander Gräwe, Jan Ranglack, Anastasia Weyrich and Viktor Stein10.Enzyme-DNA chimeras: Construction, allostery, applicationsChiao-Yu Tseng, Yong Wang and Giovanni Zocchi11.The Spy that links: Creation of nonlinear protein architectures and materials using SpyTag/SpyCatcher chemistryRi Wang and Fei Sun