Mengtao Sun obtained his Ph.D. in 2003 from the State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS). From 2003 to 2006, he worked as a postdoc at the Department of Chemical Physics, Lund University. Since 2006, as an Associate Professor, he has worked at the Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, CAS. In 2016, he became a Full Professor in University of Science and Technology Beijing, China. His current research interests focus on one and two photon absorptions in organic molecules, two dimensional (2D) materials and plasmonics, as well as the exciton-plasmon coupling interaction for molecular spectroscopy and surface catalytic reaction.Xijiao Mu, Ph D graduated from University of Science and Technology Beijing in 2022. He is Research Scientist in College of chemistry and chemical engineering, Lanzhou University. Rui Li, Associate Professor in School ofPhysics, Dalian University of Technology. Her research interest is ultrafast spectroscopy and images.

• Chapter 1 Introduction.- Chapter 2 Basic theory of nonlinear optics.- Chapter 3 Basic theory and experiment technology of CARS.- Chapter 4 CARS spectroscopy and imaging application.- Chapter 5 Basic theory and experiment technology of SRS.- Chapter 6 SRS spectroscopy and imaging application.- Chapter 7 Theory and experiment technology of TPA, TPEF and TPIF.- Chapter 8 TPA, TPEF and TPIF spectroscopy and imaging application.- Chapter 9 Theory and experiment technology of SHG.- Chapter 10 Applications of SHG imaging microscopy.