Jesper Glückstad is Professor and Group Leader at the Department of Photonics Engineering at the Technical University of Denmark. He established the Programmable Phase Optics Laboratory in Denmark in the late 90es, and served as Guest Professor in Biophotonics at Lund Institute of Technology, Sweden, 2006-2011. In 2004 he received the prestigious Doctor of Science (DSc) degree from the Technical University of Denmark. Prior to his achievements in Denmark, he was a visiting scientist at Hamamatsu Photonics Central Research Laboratories and in the Physics Dept. at Osaka University in Japan. He is the year 2000 recipient of the Danish Optical Society Award and was elected as Scientist of the Year in 2005 by Dir. Ib Henriksen’s Foundation in Denmark. Prof. Glückstad is a 2010 elected Fellow of the OSA and a Fellow of the SPIE as the first from Denmark. Between 2012 and 2014, he served on the prestigious SPIE Fellows committee. Since he obtained his PhD at the Niels Bohr Institute in 1994, he has published more than 300 journal articles and international conference papers among them several in Nature journals. He holds more than 30 international patent inventions to his name and is founder of the 2011 spin-out OptoRobotix ApS and its recent associated tech-transfer unit GPC Photonics. Darwin Palima is Associate Professor in the Department of Photonics Engineering at the Technical University of Denmark where he teaches a course he created on Biophotonics and Optical engineering. He has pioneered new scientific directions at the programmable phase optics laboratory and, as lab responsible, closely mentors the group’s PhD students and postdocs. He collaborates extensively with Jesper Glückstad from joint supervision of PhD students to joint authorships of scientific papers, conference presentations, patent applications, and popular articles. Having authored a physics textbook before moving to Denmark, he played a key role in their jointly authored monograph on Generalized Phase Contrast. The present volume is another product of their collaboration. Darwin concurrently taught at the Philippine Science High School when he worked through his PhD at the University of the Philippines proposing improvements to computer-generated holography. His interest in structured light started in the mid-90s when he studied optical vortices for his BSc thesis.

• PART 1: ELEMENTS OF LIGHT ROBOTICS1. Human gesture recognition for optical manipulation and its future nanobiophotonics applications2. Laser-based assembler and microfluidic applications3. Opto-mechanical micro-tools & shape-induced forces4. Optically-driven rotating micromachines5. Electromagnetic wave enabled micro/nano-robotic devices and their applicationsPART 2: EXPANDING POSSIBILITIES6. Complex polymer microtools for on-demand contact-free applications7. Optically Driven Micro- and Nano- Robots8. Enhancement of the second-harmonic generation in lithium niobate nanowires for localized light sources9. Next Generation Light RoboticsPART 3: BIOPHOTONICS APPLICATIONS10. Optical techniques and microtools for sub-cellular delivery and sampling 11. Optically controlled and selective fusion of cells and vesicles using plasmonic nano-heaters12. The application of optically trapped force probes in single molecule biology experiments13. Application of optical tweezers for biochemical and thermal cell stimulation14. Controlling autonomous nanobiorobots by optical micromanipulation

Jesper Glückstad is Professor and Group Leader at the Department of Photonics Engineering at the Technical University of Denmark. He established the Programmable Phase Optics Laboratory in Denmark in the late 90es, and served as Guest Professor in Biophotonics at Lund Institute of Technology, Sweden, 2006-2011. In 2004 he received the prestigious Doctor of Science (DSc) degree from the Technical University of Denmark. Prior to his achievements in Denmark, he was a visiting scientist at Hamamatsu Photonics Central Research Laboratories and in the Physics Dept. at Osaka University in Japan. He is the year 2000 recipient of the Danish Optical Society Award and was elected as Scientist of the Year in 2005 by Dir. Ib Henriksen’s Foundation in Denmark. Prof. Glückstad is a 2010 elected Fellow of the OSA and a Fellow of the SPIE as the first from Denmark. Between 2012 and 2014, he served on the prestigious SPIE Fellows committee. Since he obtained his PhD at the Niels Bohr Institute in 1994, he has published more than 300 journal articles and international conference papers among them several in Nature journals. He holds more than 30 international patent inventions to his name and is founder of the 2011 spin-out OptoRobotix ApS and its recent associated tech-transfer unit GPC Photonics. Darwin Palima is Associate Professor in the Department of Photonics Engineering at the Technical University of Denmark where he teaches a course he created on Biophotonics and Optical engineering. He has pioneered new scientific directions at the programmable phase optics laboratory and, as lab responsible, closely mentors the group’s PhD students and postdocs. He collaborates extensively with Jesper Glückstad from joint supervision of PhD students to joint authorships of scientific papers, conference presentations, patent applications, and popular articles. Having authored a physics textbook before moving to Denmark, he played a key role in their jointly authored monograph on Generalized Phase Contrast. The present volume is another product of their collaboration. Darwin concurrently taught at the Philippine Science High School when he worked through his PhD at the University of the Philippines proposing improvements to computer-generated holography. His interest in structured light started in the mid-90s when he studied optical vortices for his BSc thesis.

• PART 1: ELEMENTS OF LIGHT ROBOTICS1. Human gesture recognition for optical manipulation and its future nanobiophotonics applications2. Laser-based assembler and microfluidic applications3. Opto-mechanical micro-tools & shape-induced forces4. Optically-driven rotating micromachines5. Electromagnetic wave enabled micro/nano-robotic devices and their applicationsPART 2: EXPANDING POSSIBILITIES6. Complex polymer microtools for on-demand contact-free applications7. Optically Driven Micro- and Nano- Robots8. Enhancement of the second-harmonic generation in lithium niobate nanowires for localized light sources9. Next Generation Light RoboticsPART 3: BIOPHOTONICS APPLICATIONS10. Optical techniques and microtools for sub-cellular delivery and sampling 11. Optically controlled and selective fusion of cells and vesicles using plasmonic nano-heaters12. The application of optically trapped force probes in single molecule biology experiments13. Application of optical tweezers for biochemical and thermal cell stimulation14. Controlling autonomous nanobiorobots by optical micromanipulation

Jesper Glückstad is Professor and Group Leader at the Department of Photonics Engineering at the Technical University of Denmark. He established the Programmable Phase Optics Laboratory in Denmark in the late 90es, and served as Guest Professor in Biophotonics at Lund Institute of Technology, Sweden, 2006-2011. In 2004 he received the prestigious Doctor of Science (DSc) degree from the Technical University of Denmark. Prior to his achievements in Denmark, he was a visiting scientist at Hamamatsu Photonics Central Research Laboratories and in the Physics Dept. at Osaka University in Japan. He is the year 2000 recipient of the Danish Optical Society Award and was elected as Scientist of the Year in 2005 by Dir. Ib Henriksen’s Foundation in Denmark. Prof. Glückstad is a 2010 elected Fellow of the OSA and a Fellow of the SPIE as the first from Denmark. Between 2012 and 2014, he served on the prestigious SPIE Fellows committee. Since he obtained his PhD at the Niels Bohr Institute in 1994, he has published more than 300 journal articles and international conference papers among them several in Nature journals. He holds more than 30 international patent inventions to his name and is founder of the 2011 spin-out OptoRobotix ApS and its recent associated tech-transfer unit GPC Photonics. Darwin Palima is Associate Professor in the Department of Photonics Engineering at the Technical University of Denmark where he teaches a course he created on Biophotonics and Optical engineering. He has pioneered new scientific directions at the programmable phase optics laboratory and, as lab responsible, closely mentors the group’s PhD students and postdocs. He collaborates extensively with Jesper Glückstad from joint supervision of PhD students to joint authorships of scientific papers, conference presentations, patent applications, and popular articles. Having authored a physics textbook before moving to Denmark, he played a key role in their jointly authored monograph on Generalized Phase Contrast. The present volume is another product of their collaboration. Darwin concurrently taught at the Philippine Science High School when he worked through his PhD at the University of the Philippines proposing improvements to computer-generated holography. His interest in structured light started in the mid-90s when he studied optical vortices for his BSc thesis.

• PART 1: ELEMENTS OF LIGHT ROBOTICS1. Human gesture recognition for optical manipulation and its future nanobiophotonics applications2. Laser-based assembler and microfluidic applications3. Opto-mechanical micro-tools & shape-induced forces4. Optically-driven rotating micromachines5. Electromagnetic wave enabled micro/nano-robotic devices and their applicationsPART 2: EXPANDING POSSIBILITIES6. Complex polymer microtools for on-demand contact-free applications7. Optically Driven Micro- and Nano- Robots8. Enhancement of the second-harmonic generation in lithium niobate nanowires for localized light sources9. Next Generation Light RoboticsPART 3: BIOPHOTONICS APPLICATIONS10. Optical techniques and microtools for sub-cellular delivery and sampling 11. Optically controlled and selective fusion of cells and vesicles using plasmonic nano-heaters12. The application of optically trapped force probes in single molecule biology experiments13. Application of optical tweezers for biochemical and thermal cell stimulation14. Controlling autonomous nanobiorobots by optical micromanipulation