Laboratory Protocols in Fungal Biology

Editors Vijai Kumar Gupta is Assistant Professor of Biotechnology at MITS University, India. Presently, he is working as a Post Doctoral Research Scientist, at NUIG, Ireland. He obtained his Ph.D. in Microbiology from Dr. R.M.L. Avadh University, India. He has been honoured with several awards, including the prestigious Indian ICAR Senior Research Fellowship and Young Scientist Award-2009, 2011. He has over seven years of research experience in Applied Microbiology and Biotechnology with a special interest in fungal biology. He has edited books published from Lambert Academic Publishing, Germany; Nova Science Publishers, USA; Science Publisher, CRC Press, Taylor & Francis, USA; and Springer, USA. Dr. Maria G. Tuohy is the Head of the Molecular Glycobiotechnology Research Group, NUI Galway. She has more than 20 years experience in the molecular biochemistry, genetics and biotechnology of fungi, with a special interest in thermophilic ascomycetes and the characterization of these fungi as cell factories for protein production, including novel thermostable enzymes/enzyme systems. Dr. Tuohy has authored 132 research publications. She is also a reviewer for international journals and funding agencies, an associate editor for Microbiology and co-editor of four books with Dr. V. K. Gupta. Associate Editors Dr. Manimaran Ayyachamy is a Research Scientist with NUIG, Ireland. He obtained his PhD in Microbiology from the University of Madras, India. He has twelve years of research experience in microbial biotechnology with a special interest in microbial enzymes and biofuels technology. He has co-edited books published from Lambert Academic Publishing, Germany; Nova Science Publishers, USA; Science Publisher, CRC Press, Taylor & Francis, USA; and Springer, USA with Dr. V.K. Gupta Dr. Kevin M. Turner is a Research Scientist in the division of Manufacturing Sciences and Technology in Pfizer Ireland Pharmaceuticals, Dublin. He obtained his PhD from the University of Limerick, Ireland. He has three years of research experience in microbial biotechnology. Dr. Anthonia O'Donovan is a Postdoctoral Research Scientist with NUIG, Ireland. She obtained her PhD in Biochemistry from the National University of Ireland Galway, Ireland. She has seven years of research experience in microbial enzymes and biofuels technology.

• Safety Norms and Regulations in Handling Fungal Specimens.- Methods of Cryopreservation in Fungi.- Long Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results.- Fungal Specimen Collection and Processing.- Chemical and Molecular Methods for Detection of Toxigenic Fungi and Their Mycotoxins from Major Food Crops.- Identification Key for the Major Growth Forms of Lichenized Fungi.- Microscopic Methods for Analytical Studies of Fungi.- Scanning Electron Microscopy for Fungal Sample Examination.- High Resolution Imaging and Force Spectroscopy of Fungal Hyphal Cells by Atomic Force Microscopy.- Use of Fourier-Transform Infrared (FTIR) Microscopy Method For Detection of Phyto-Fungal Pathogens.- Diagnose of Parasitic Fungi in The Plankton:  Technique for Identifying And Counting Infective Chytrids Using Epifluorescence Microscopy.- Fungal Cell Wall Analysis.- Histopathological Technique for Detection of Fungal Infections in Plants.- Development of Media for Growth and Enumeration of Fungi from Water.- Sabouraud Agar for Fungal Growth.- A Method for the Formation of Candida Biofilms in 96 Well Microtiter Plates and its Application to Antifungal Susceptibility Testing.- Screening for Compounds Exerting Antifungal Activities.- Fluorescence In situ Hybridization of Uncultured Zoosporic Fungi.- Staining Techniques and Biochemical Methods for the Identification of Fungi.- Protocol for the In vivo Quantification of Superoxide Radical in Fungi.- Isolation of Intact RNA from Sorted S. cerevisiae Cells for Differential Gene Expression Analysis.- Quantitative PCR Analysis of Double-Stranded RNA-Mediated Gene Silencing in Fungi.- Semi-nested PCR Approach to Amplify Large 8S rRNA Gene Fragments for PCR-DGGE Analysis of Soil Fungal Communities.- Proteomic Protocols for the Study of Filamentous Fungi.- Detection and Quantification of Endoprotease Activity Using a Coomassie Dye-Binding Assay.- Protocol of a LightCycler PCR Assay for Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples of Neutropenic Patients.- Application of Polymerase Chain Reaction (PCR) and PCR Based Methods Targeting Internal Transcribed Spacer (ITS) for Detection and Species Level Identification of Fungi.- Real-Time PCR Assay in Fungi.- Quantitative Sampling Methods for the Analysis of Fungi: Air Sampling.- Transformation of Filamentous Fungi in Microtiter Plate.- Molecular Fingerprinting of Fungal Communities in Soil.- Development of Microsatellite Markers from Fungal DNA Based on Shotgun Pyrosequencing.- Multiplex and Quantifiable Detection of Infectious Fungi Using Padlock Probes, General qPCR and Suspension Microarray Readout.- Rapid Deletion Plasmid Construction Methods for Protoplast and Agrobacterium-based Fungal Transformation Systems.- Improved Transformation Method for Alternaria brassicicola and its Applications.- Methods for High Quality DNA Extraction from Fungi.- Production of Recombinant Proteins from Pichia pastoris: Interfacing Fermentation and Immobilized Metal Ion Affinity Chromatography.- Development of a Real-Time Quantitative PCR Assay for the Assessment of Uncultured Zoosporic Fungi.- Nucleic and Protein Extraction Methods for Fungal Exopolysaccharide Producers.- Directed Evolution of A Fungal Xylanase for Improvement of Thermal and Alkaline Stability.- Genome Shuffling Protocol for the Pentose-Fermenting Yeast Scheffersomyces stipitis.- Detection and Identification of Fungal Microbial Volatile Organic Compounds by HS-SPME-GC-MS.- Transformation Methods for Slow Growing Fungi.- Enzymatic Saccharification of lignocellulosic biomass.- Protoplast Fusion Techniques in Fungi.- Large Scale Production of Lignocellulolytic Enzymes in Thermophilic Fungi.- Panfungal PCR Method for Detection of Aflatoxigenic Molds.- Protocols for the Quantification of dsDNA and Its Fragmentation Status in Fungi.- Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes.- Drug-Induced Permeabilization in Fungi.- Extraction and Characterization of Taxol: An Anticancer Drug from an Endophytic And Pathogenic Fungi.- Identification of Mycotoxigenic Fungi using an Oligonucleotide Microarray.- DNA Microarray-Based Detection and Identification of Fungal Specimens.- Bioinformatic Protocols and the Knowledge-Base for Secretomes in Fungi.- High-Throughput Functional Annotation and Data Mining of Fungal Genomes to Identify Therapeutic Targets.- Application of Support Vector Machines in Fungal Genome and Proteome Annotation.- Bioinformatics Tools for the Multilocus Phylogenetic Analysis of Fungi.

From the book reviews: "The book 'Laboratory Protocols in Fungal Biology' written by international researchers, offers diverse modern laboratory techniques in the field of fungal biology. ... Each chapter is comprehensive, provides readily reproducible protocols and is easy to grasp by beginners. This book is a valuable guide to budding mycologists as well as professionals. It is recommended to all the Universities. A crucial Laboratory Manual for Fungal Biologists, Taxonomists, Fungal Biotechnologists, Biochemists, Histochemists, Plant Pathologists and Laboratory technicians." (Melvina D'souza and Samantha C. Karunarathna, Fungal Diversity, December, 2014) "This new book is very much hands-on, and covers an extraordinarily diverse range of topics. ... Chapters are well-referenced to primary literature, and there are numerous links to website. There are numerous illustrations, most clearly presented, and in some cases using colour. This is a reference work that deserves to be accessible in all mycology laboratories ... ." (IMA Fungus, Vol. 4 (1), 2013)