Del i serien Foundations and Frontiers in Enzymology

Isothermal Titration Calorimetry in Enzymology

Techniques and Applications

Häftad, Engelska, 2025

2 119 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt för medlemmar vid köp för minst 249 kr.

Isothermal Titration Calorimetry in Enzymology: Techniques and Applications provides a thorough, practical overview of ITC as a productive and powerful tool for quantifying enzyme catalysis and interactions with substrates, products, cofactors, and inhibitors. Here, global experts in the methodology discuss the full capabilities of ITC and how it may be applied in research, medicine, and industry and offer a basis to continue leveraging ITC as its uses expand and evolve. This book bridges the gap between research and application by giving extensive technical details on cutting edge ITC techniques across a wide swath of both ITC kinetics measurements and ITC thermodynamic measurements, alongside case studies illustrating the depth of knowledge that can be obtained. Applications discussed include measuring binding kinetics by ITC, analyzing ITC kinetic data, in vivo enzyme kinetics by ITC, analyzing enzyme kinetics in crowded solutions, studying catalysis of biological substrates, interpreting ITC binding curves, ITC in drug discovery, ITC in the development of cancer therapeutics, ITC analysis of aminoglycoside resistance enzymes, studying enzyme interactions with toxic metals, using ITC to study ubiquitination, and ITC studies of glycoside hydrolases, among others.

Describes and instructs in cutting-edge ITC experiments and analysis techniques
Covers both kinetics and thermodynamics-based ITC approaches
Features illustrative case studies across a wide range of applications
Includes chapter contributions from international experts in ITC

Produktinformation

Utgivningsdatum2025-10-27
Mått191 x 235 x 21 mm
Vikt740 g
FormatHäftad
SpråkEngelska
SerieFoundations and Frontiers in Enzymology
Antal sidor354
FörlagElsevier Science
ISBN9780443218484

Tillhör följande kategorier

Tillämpad fysik inom Naturvetenskap och teknik
Biokemi inom Naturvetenskap och teknik

PART 1: ITC kinetics measurements1. Enzyme kinetics by ITC2. Measuring binding kinetics by ITC3. Analysing ITC kinetic data with AFFinimeter software4. In vivo enzyme kinetics by ITC5. Characterizing enzyme kinetics by 2D-ITC6. Measuring enzyme stability using ITC7. Kinetics of immobilized enzymes8. Enzyme kinetics in crowded solutions9. Kinetics of nanozymes10. Using ITC to characterize enzyme allostery11. Signal amplification in ITC kinetics experiments12. ITC kinetic studies of 6-phytase13. Catalysis of biological substrates of nuclear metallohydrolases14. Alpha-amylase activity and inhibition15. Enzyme stability and activity of psycrophillic enzymes16. Non-MM kinetics of peroxidases and application to industrial wastes17. Kinetics of diphosphohydrolasesPART 2: ITC thermodynamic measurements18. Interpretation of ITC binding curves19. Analysis of ITC binding data using SEDPHAT20. ITC in drug discovery21. ITC in the development of cancer therapeutics22. Mechanisms of aminoglycoside resistance enzymes23. Using ITC to characterize the interactions of natural products with biomolecules24. ITC studies of metalloenzymes25. Enzyme interactions with toxic metals26. Thermodynamics of molecular machines27. Thermodynamics of protein chaperones28. Characterizing protein interactions in apoptosis by ITC29. Using ITC to study ubiquitination30. Thermodynamics of transition state analogs31. Thermodynamics of recombinant enzymes in biotechnology32. Ion Binding to Transport Proteins using ITC33. Urease from Helicobacter pylori, lessons from ITC34. Substrate recognition by Chitinases35. ITC studies of glycoside hydrolases

Du kanske också är intresserad av

Sustainable Process Intensification for Biocatalysis

Ganapati D. Yadav, Australia) Yadav, Ganapati D. (Emeritus Professor of Eminence, J.C. Bose National Fellow (Govt. of India); Adjunct Professor, University of Saskatchewan, Canada; Conjoint Professor, University of New Castle

Häftad

2 139 kr