Department of Cell Biology, The Johns Hopkins University School of Medicine, USA Department of Cell Biology, The Netherlands Cancer Institute, The Netherlands

• Part I: Identification and Biochemical Analysis of Lamin-Associated Proteins 1. BioID Identification of Lamin-Associated ProteinsAaron A. Mehus, Ruthellen H. Anderson and Kyle J. Roux2. Proximity-Dependent Biotin Identification (BioID) in Dictyostelium AmoebaePetros Batsios, Irene Meyer and Ralph Gräf3. Purification and Structural Analysis of LEM-Domain ProteinsIsaline Herrada, Benjamin Bourgeois, Camille Samson, Brigitte Buendia, Howard J Worman and Sophie Zinn-Justin4. Purification and Structural Analysis of SUN and KASH Domain ProteinsF. Esra Demircioglu, Victor E. Cruz and Thomas U. Schwartz5. Purification of Lamins and Soluble Fragments of NETsAlexandr A. Makarov, Andrea Rizzotto, Peter Meinke and Eric C. Schirmer6. Simple Separation of Functionally-Distinct Populations of Lamin-Binding ProteinsJason M. Berk and Katherine L. WilsonPart II: General Methods for Analysis of Intermediate Filament-Associated Proteins 7. Development of a Novel Green Fluorescent Protein-Based Binding Assay to Study the Association of Plakins with Intermediate Filament ProteinsBertrand Favre, Nadja Begré, Jamal-Eddine Bouameur, and Luca Borradori8. Functional Analysis of Keratin-Associated Proteins in Intestinal Epithelia: Heat-  Shock Protein Chaperoning and Kinase RescueAnastasia Mashukova, Radia Forteza and Pedro J. Salas9. Purification of Protein Chaperones and their Functional Assays with Intermediate FilamentsMing Der Perng, Yu-Shan Huang and Roy A. Quinlan10. Purification and Structural Analysis of Plectin and BPAG1eJosé A Manso, Inés García Rubio, María Gómez-Hernández, Esther Ortega, Rubén M  Buey, Ana M Carballido, Arturo Carabias, Noelia Alonso-García and José M de Pereda 11. Purification and Structural Analysis of DesmoplakinHee-Jung Choi and William I. Weis12. Degradation of the Intermediate Filament Family by GigaxoninPascale BomontPart III: Functional and Genetic Analysis of the Plakin and Other Cytoskeletal Cross-Linkers 13. Functional and Genetic Analysis of Plectin in Skin And MuscleGünther A. Rezniczek, Lilli Winter, Gernot Walko, and Gerhard Wiche14. Functional and Genetic Analysis of Epiplakin in Epithelial CellsSandra Szabo, Karl L. Wögenstein and Peter Fuchs15. In Vitro Model of the Epidermis: Connecting Protein Function to 3D StructureChristopher Arnette,Jennifer L. Koetsier, Paul Hoover, Spiro Getsios and Kathleen J.     Green16. Functional Analysis of Periplakin and Envoplakin, Cytoskeletal Linkers and Cornified Envelope Precursor ProteinsVeronika Boczonadi and Arto Määttä17. Microtubule-Actin Crosslinking Factor 1: Domains, Interaction Partners and Tissue-Specific FunctionsDmitry Goryunov and Ronald K. H. Liem18. Functional and Genetic Analysis of Neuronal Isoforms of BPAG1Anisha Lynch-Godrei and Rashmi Kothary19. "Functional and Genetic Analysis of Spectraplakins in Drosophila”Ines Hahn, Matthew Ronshaugen, Natalia Sánchez-Soriano and Andreas Prokop20. Functional and Genetic Analysis of VAB-10 Spectraplakin in C. ElegansChristelle Gally, Huimin Zhang and Michel LabouessePart IV: Functional and Genetic Analysis of Lamin-associated proteins21. Tagged Chromosomal Insertion Site (TCIS) System, a Method to Study Lamina  Associated ChromatinJennifer C. Harr and Karen L. Reddy22. Lamin Binding Proteins in Caenorhabditis ElegansAgnieszka Dobrzynska, Peter Askjaer and Yosef Gruenbaum23. Pathologically-Relevant Prelamin A Interactions with Transcription FactorsArantza Infante and Clara I. Rodríguez24. MCLIP Detection of Novel Protein-Protein Interactions at the Nuclear EnvelopeMohammed Hakim Jafferali, Ricardo A. Figueroa and Einar Hallberg25. Preparation of the Human Cytomegalovirus Nuclear Egress Complex and Associated ProteinsMayuri Sharma, Jeremy P. Kamil and Donald M. Coen

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