Reiner Salzer is Professor Emeritus of Analytical Chemistry at the Dresden University of Technology (Germany). He has authored almost 300 publications and serves on various national and international scientific boards. Professor Salzer is a member of the Norwegian Academy of Science and a recipient of the Emich Plaque of the Austrian Society of Analytical Chemistry. Recently he also received the Clemens Winkler Medal of the GDCh (German Chemical Society), the Hanus Medal of the Czech Chemical Society and the Ioannes Marcus Marci Medal of the Czech Spectroscopic Society. Professor Salzer served as President of the Division Analytical Chemistry of the GDCh and is National Delegate to the Division Analytical Chemistry of EuCheMS, where he is Head of the Study Group Education. He is Chairman of the ECTN Label Committee for the Quality Eurolabels in Chemistry Education. Heinz W. Siesler is Professor Emeritus of Physical Chemistry at the University of Duisburg-Essen (Germany). After receiving his PhD in chemistry from the University of Vienna (Austria), he worked as a postdoctoral fellow at the University of Cologne (Germany) and as a lecturer at the Witwatersrand University (Johannesburg, South Africa). Prior to his academic position, he gained industrial experience as sectionhead in molecular spectroscopy and thermal analysis in the Corporate R&D Department of Bayer AG (Dormagen, Germany). Between 1992 and 2010 he held guest professorships in France, Japan, and Austria. Professor Siesler is a recipient of the EAS Award, the Tomas Hirschfeld Award and the Buechi Award in near-infrared spectroscopy and he is a Fellow of the Society for Applied Spectroscopy. His main research focuses on the application of vibrational spectroscopy to chemical and polymer research, analysis and quality control, and he has authored more than 230 publications in this field.

• Preface PART I: Basic Methodology INFRARED AND RAMAN INSTRUMENTATION FOR MAPPING AND IMAGINGIntroduction to Mapping and ImagingMid-Infrared Microspectroscopy and MappingRaman Microspectroscopy and MappingNear-Infrared Hyperspectral ImagingRaman Hyperspectral ImagingMid-Infrared Hyperspectral ImagingMapping with Pulsed Terahertz RadiationSummary CHEMOMETRIC TOOLS FOR IMAGE ANALYSISIntroductionHyperspectral Images: The MeasurementImage PreprocessingExploratory Image AnalysisQuantitative Image Information: Multivariate Image Regression (MIR)Image SegmentationImage ResolutionFuture Trends PART II: Biomedical Applications VIBRATIONAL SPECTROSCOPIC IMAGING OF SOFT TISSUEIntroductionPreparation of Soft Tissue for Vibrational Spectroscopic ImagingApplications to Soft TissueConclusions VIBRATIONAL SPECTROSCOPIC ANALYSIS OF HARD TISSUESIntroductionImportance of Tissue Age versus Specimen AgeFT-IR SpectroscopyRaman SpectroscopyClinical Applications of Raman Spectroscopy MEDICAL APPLICATIONS OF INFRARED SPECTRAL IMAGING OF INDIVIDUAL CELLSIntroductionMethodsResults and DiscussionFuture Potential of SCP/Conclusions PART III: Agriculture, Plants, and Food INFRARED AND RAMAN SPECTROSCOPIC MAPPING AND IMAGING OF PLANT MATERIALSIntroduction, Background, and PerspectiveApplication of Mapping and Imaging to Horticultural CropsApplication of Mapping and Imaging to Agricultural CropsMapping and Imaging of Wild Plants and TreesApplication of Mapping and Imaging to AlgaeInteraction Between Plant Tissue and Plant Pathogens NIR HYPERSPECTRAL IMAGING FOR FOOD AND AGRICULTURAL PRODUCTSIntroductionHSI as a "Super" NIR AnalyzerNIR HS Imager as a "Super" Vision SystemConclusion PART IV: Polymers and Pharmaceuticals FT-IR AND NIR SPECTROSCOPIC IMAGING: PRINCIPLES, PRACTICAL ASPECTS, AND APPLICATIONS IN MATERIAL AND PHARMACEUTICAL SCIENCEIntroductionInstrumentation for NIR and FT-IR ImagingApplications of FT-IR and FT-NIR Imaging for Polymer CharacterizationNIR Imaging Spectroscopy for Quality Control of Pharmaceutical Drug FormulationsFT-IR Spectroscopic Imaging of Inorganic Materials FT-IR IMAGING IN ATR AND TRANSMISSION MODES: PRACTCAL CONSIDERATIONS AND EMERGING APPLICATIONSFT-IR Imaging: IntroductionFT-IR Imaging: Technical ConsiderationsPractical ApplicationsConclusion and Outlook TERAHERTZ IMAGING OF DRUG PRODUCTSIntroductionLow Wavenumber Region in the Infrared SpectrumTHz-TDS Technology and ApplicationsTHz Imaging in the Pharmaceutical IndustryGoing ForwardCompetition versus Cost: A Challenge for the FutureConclusion PART V: Imaging Beyond the Diffraction Limit SPECTROSCOPIC IMAGING OF BIOLOGICAL SAMPLES USING NEAR-FIELD METHODSTip-Enhanced Raman Scattering (TERS)Detection of BiomoleculesBiopolymersMembranes, Viruses, and BacteriaConclusion INFRARED MAPPING BELOW THE DIFFRACTION LIMITIntroduction and Description of Early WorkNear-Field Microscopy by Elastic Scattering from a Tip PART VI: Developments in Methodology SUBSURFACE RAMAN SPECTROSCOPY IN TURBID MEDIAIntroductionTechniques for Deep Noninvasive Raman SpectroscopyExamples of Application AreasConclusions NONLINEAR VIBRATIONAL SPECTROSCOPIC MICROSCOPY OF CELLS AND TISSUEIntroductionPrinciples of Nonlinear Optical ImagingInstrumentation for Multimodal Nonlinear MicroscopyApplications WIDEFIELD FT-IR 2D AND 3D IMAGING AT THE MICROSCALE USING SYNCHROTRON RADIATIONIntroductionOptical EvaluationMathematical Evaluation of Hyperspectral CubesWidefield versus Raster Scanning GeometriesExamplesConclusions Index

“This book is an excellent addition to the bookshelves of vibrational spectroscopic imaging practitioners as well as researchers in related fields, including biology, medicine, surface analysis, process control, forensics, and others.”  (Anal Bioanal Chem, 10 May 2015)

Reiner Salzer is Professor Emeritus of Analytical Chemistry at the Dresden University of Technology (Germany). He has authored almost 300 publications and serves on various national and international scientific boards. Professor Salzer is a member of the Norwegian Academy of Science and a recipient of the Emich Plaque of the Austrian Society of Analytical Chemistry. Recently he also received the Clemens Winkler Medal of the GDCh (German Chemical Society), the Hanus Medal of the Czech Chemical Society and the Ioannes Marcus Marci Medal of the Czech Spectroscopic Society. Professor Salzer served as President of the Division Analytical Chemistry of the GDCh and is National Delegate to the Division Analytical Chemistry of EuCheMS, where he is Head of the Study Group Education. He is Chairman of the ECTN Label Committee for the Quality Eurolabels in Chemistry Education. Heinz W. Siesler is Professor Emeritus of Physical Chemistry at the University of Duisburg-Essen (Germany). After receiving his PhD in chemistry from the University of Vienna (Austria), he worked as a postdoctoral fellow at the University of Cologne (Germany) and as a lecturer at the Witwatersrand University (Johannesburg, South Africa). Prior to his academic position, he gained industrial experience as sectionhead in molecular spectroscopy and thermal analysis in the Corporate R&D Department of Bayer AG (Dormagen, Germany). Between 1992 and 2010 he held guest professorships in France, Japan, and Austria. Professor Siesler is a recipient of the EAS Award, the Tomas Hirschfeld Award and the Buechi Award in near-infrared spectroscopy and he is a Fellow of the Society for Applied Spectroscopy. His main research focuses on the application of vibrational spectroscopy to chemical and polymer research, analysis and quality control, and he has authored more than 230 publications in this field.

• Preface PART I: Basic Methodology INFRARED AND RAMAN INSTRUMENTATION FOR MAPPING AND IMAGINGIntroduction to Mapping and ImagingMid-Infrared Microspectroscopy and MappingRaman Microspectroscopy and MappingNear-Infrared Hyperspectral ImagingRaman Hyperspectral ImagingMid-Infrared Hyperspectral ImagingMapping with Pulsed Terahertz RadiationSummary CHEMOMETRIC TOOLS FOR IMAGE ANALYSISIntroductionHyperspectral Images: The MeasurementImage PreprocessingExploratory Image AnalysisQuantitative Image Information: Multivariate Image Regression (MIR)Image SegmentationImage ResolutionFuture Trends PART II: Biomedical Applications VIBRATIONAL SPECTROSCOPIC IMAGING OF SOFT TISSUEIntroductionPreparation of Soft Tissue for Vibrational Spectroscopic ImagingApplications to Soft TissueConclusions VIBRATIONAL SPECTROSCOPIC ANALYSIS OF HARD TISSUESIntroductionImportance of Tissue Age versus Specimen AgeFT-IR SpectroscopyRaman SpectroscopyClinical Applications of Raman Spectroscopy MEDICAL APPLICATIONS OF INFRARED SPECTRAL IMAGING OF INDIVIDUAL CELLSIntroductionMethodsResults and DiscussionFuture Potential of SCP/Conclusions PART III: Agriculture, Plants, and Food INFRARED AND RAMAN SPECTROSCOPIC MAPPING AND IMAGING OF PLANT MATERIALSIntroduction, Background, and PerspectiveApplication of Mapping and Imaging to Horticultural CropsApplication of Mapping and Imaging to Agricultural CropsMapping and Imaging of Wild Plants and TreesApplication of Mapping and Imaging to AlgaeInteraction Between Plant Tissue and Plant Pathogens NIR HYPERSPECTRAL IMAGING FOR FOOD AND AGRICULTURAL PRODUCTSIntroductionHSI as a "Super" NIR AnalyzerNIR HS Imager as a "Super" Vision SystemConclusion PART IV: Polymers and Pharmaceuticals FT-IR AND NIR SPECTROSCOPIC IMAGING: PRINCIPLES, PRACTICAL ASPECTS, AND APPLICATIONS IN MATERIAL AND PHARMACEUTICAL SCIENCEIntroductionInstrumentation for NIR and FT-IR ImagingApplications of FT-IR and FT-NIR Imaging for Polymer CharacterizationNIR Imaging Spectroscopy for Quality Control of Pharmaceutical Drug FormulationsFT-IR Spectroscopic Imaging of Inorganic Materials FT-IR IMAGING IN ATR AND TRANSMISSION MODES: PRACTCAL CONSIDERATIONS AND EMERGING APPLICATIONSFT-IR Imaging: IntroductionFT-IR Imaging: Technical ConsiderationsPractical ApplicationsConclusion and Outlook TERAHERTZ IMAGING OF DRUG PRODUCTSIntroductionLow Wavenumber Region in the Infrared SpectrumTHz-TDS Technology and ApplicationsTHz Imaging in the Pharmaceutical IndustryGoing ForwardCompetition versus Cost: A Challenge for the FutureConclusion PART V: Imaging Beyond the Diffraction Limit SPECTROSCOPIC IMAGING OF BIOLOGICAL SAMPLES USING NEAR-FIELD METHODSTip-Enhanced Raman Scattering (TERS)Detection of BiomoleculesBiopolymersMembranes, Viruses, and BacteriaConclusion INFRARED MAPPING BELOW THE DIFFRACTION LIMITIntroduction and Description of Early WorkNear-Field Microscopy by Elastic Scattering from a Tip PART VI: Developments in Methodology SUBSURFACE RAMAN SPECTROSCOPY IN TURBID MEDIAIntroductionTechniques for Deep Noninvasive Raman SpectroscopyExamples of Application AreasConclusions NONLINEAR VIBRATIONAL SPECTROSCOPIC MICROSCOPY OF CELLS AND TISSUEIntroductionPrinciples of Nonlinear Optical ImagingInstrumentation for Multimodal Nonlinear MicroscopyApplications WIDEFIELD FT-IR 2D AND 3D IMAGING AT THE MICROSCALE USING SYNCHROTRON RADIATIONIntroductionOptical EvaluationMathematical Evaluation of Hyperspectral CubesWidefield versus Raster Scanning GeometriesExamplesConclusions Index

“This book is an excellent addition to the bookshelves of vibrational spectroscopic imaging practitioners as well as researchers in related fields, including biology, medicine, surface analysis, process control, forensics, and others.”  (Anal Bioanal Chem, 10 May 2015)

Reiner Salzer is Professor Emeritus of Analytical Chemistry at the Dresden University of Technology (Germany). He has authored almost 300 publications and serves on various national and international scientific boards. Professor Salzer is a member of the Norwegian Academy of Science and a recipient of the Emich Plaque of the Austrian Society of Analytical Chemistry. Recently he also received the Clemens Winkler Medal of the GDCh (German Chemical Society), the Hanus Medal of the Czech Chemical Society and the Ioannes Marcus Marci Medal of the Czech Spectroscopic Society. Professor Salzer served as President of the Division Analytical Chemistry of the GDCh and is National Delegate to the Division Analytical Chemistry of EuCheMS, where he is Head of the Study Group Education. He is Chairman of the ECTN Label Committee for the Quality Eurolabels in Chemistry Education. Heinz W. Siesler is Professor Emeritus of Physical Chemistry at the University of Duisburg-Essen (Germany). After receiving his PhD in chemistry from the University of Vienna (Austria), he worked as a postdoctoral fellow at the University of Cologne (Germany) and as a lecturer at the Witwatersrand University (Johannesburg, South Africa). Prior to his academic position, he gained industrial experience as sectionhead in molecular spectroscopy and thermal analysis in the Corporate R&D Department of Bayer AG (Dormagen, Germany). Between 1992 and 2010 he held guest professorships in France, Japan, and Austria. Professor Siesler is a recipient of the EAS Award, the Tomas Hirschfeld Award and the Buechi Award in near-infrared spectroscopy and he is a Fellow of the Society for Applied Spectroscopy. His main research focuses on the application of vibrational spectroscopy to chemical and polymer research, analysis and quality control, and he has authored more than 230 publications in this field.

• Preface PART I: Basic Methodology INFRARED AND RAMAN INSTRUMENTATION FOR MAPPING AND IMAGINGIntroduction to Mapping and ImagingMid-Infrared Microspectroscopy and MappingRaman Microspectroscopy and MappingNear-Infrared Hyperspectral ImagingRaman Hyperspectral ImagingMid-Infrared Hyperspectral ImagingMapping with Pulsed Terahertz RadiationSummary CHEMOMETRIC TOOLS FOR IMAGE ANALYSISIntroductionHyperspectral Images: The MeasurementImage PreprocessingExploratory Image AnalysisQuantitative Image Information: Multivariate Image Regression (MIR)Image SegmentationImage ResolutionFuture Trends PART II: Biomedical Applications VIBRATIONAL SPECTROSCOPIC IMAGING OF SOFT TISSUEIntroductionPreparation of Soft Tissue for Vibrational Spectroscopic ImagingApplications to Soft TissueConclusions VIBRATIONAL SPECTROSCOPIC ANALYSIS OF HARD TISSUESIntroductionImportance of Tissue Age versus Specimen AgeFT-IR SpectroscopyRaman SpectroscopyClinical Applications of Raman Spectroscopy MEDICAL APPLICATIONS OF INFRARED SPECTRAL IMAGING OF INDIVIDUAL CELLSIntroductionMethodsResults and DiscussionFuture Potential of SCP/Conclusions PART III: Agriculture, Plants, and Food INFRARED AND RAMAN SPECTROSCOPIC MAPPING AND IMAGING OF PLANT MATERIALSIntroduction, Background, and PerspectiveApplication of Mapping and Imaging to Horticultural CropsApplication of Mapping and Imaging to Agricultural CropsMapping and Imaging of Wild Plants and TreesApplication of Mapping and Imaging to AlgaeInteraction Between Plant Tissue and Plant Pathogens NIR HYPERSPECTRAL IMAGING FOR FOOD AND AGRICULTURAL PRODUCTSIntroductionHSI as a "Super" NIR AnalyzerNIR HS Imager as a "Super" Vision SystemConclusion PART IV: Polymers and Pharmaceuticals FT-IR AND NIR SPECTROSCOPIC IMAGING: PRINCIPLES, PRACTICAL ASPECTS, AND APPLICATIONS IN MATERIAL AND PHARMACEUTICAL SCIENCEIntroductionInstrumentation for NIR and FT-IR ImagingApplications of FT-IR and FT-NIR Imaging for Polymer CharacterizationNIR Imaging Spectroscopy for Quality Control of Pharmaceutical Drug FormulationsFT-IR Spectroscopic Imaging of Inorganic Materials FT-IR IMAGING IN ATR AND TRANSMISSION MODES: PRACTCAL CONSIDERATIONS AND EMERGING APPLICATIONSFT-IR Imaging: IntroductionFT-IR Imaging: Technical ConsiderationsPractical ApplicationsConclusion and Outlook TERAHERTZ IMAGING OF DRUG PRODUCTSIntroductionLow Wavenumber Region in the Infrared SpectrumTHz-TDS Technology and ApplicationsTHz Imaging in the Pharmaceutical IndustryGoing ForwardCompetition versus Cost: A Challenge for the FutureConclusion PART V: Imaging Beyond the Diffraction Limit SPECTROSCOPIC IMAGING OF BIOLOGICAL SAMPLES USING NEAR-FIELD METHODSTip-Enhanced Raman Scattering (TERS)Detection of BiomoleculesBiopolymersMembranes, Viruses, and BacteriaConclusion INFRARED MAPPING BELOW THE DIFFRACTION LIMITIntroduction and Description of Early WorkNear-Field Microscopy by Elastic Scattering from a Tip PART VI: Developments in Methodology SUBSURFACE RAMAN SPECTROSCOPY IN TURBID MEDIAIntroductionTechniques for Deep Noninvasive Raman SpectroscopyExamples of Application AreasConclusions NONLINEAR VIBRATIONAL SPECTROSCOPIC MICROSCOPY OF CELLS AND TISSUEIntroductionPrinciples of Nonlinear Optical ImagingInstrumentation for Multimodal Nonlinear MicroscopyApplications WIDEFIELD FT-IR 2D AND 3D IMAGING AT THE MICROSCALE USING SYNCHROTRON RADIATIONIntroductionOptical EvaluationMathematical Evaluation of Hyperspectral CubesWidefield versus Raster Scanning GeometriesExamplesConclusions Index

“This book is an excellent addition to the bookshelves of vibrational spectroscopic imaging practitioners as well as researchers in related fields, including biology, medicine, surface analysis, process control, forensics, and others.”  (Anal Bioanal Chem, 10 May 2015)