This book constitutes the refereed proceedings of the 21st International Conference on Information Technologies and Mathematical Modelling. Queueing Theory and Applications, ITMM 2022, held in Karshi, Uzbekistan, during October 25–29, 2022. The 19 full papers included in this book were carefully reviewed and selected from 89 submissions. The papers are devoted to new results in queueing theory and its applications. Its target audience includes specialists in probabilistic theory, random processes, mathematical modeling as well as engineers engaged in logical and technical design and operational management of data processing systems, communication, and computer networks.