Induced Pluripotent Stem Cells and Human Disease

Methods and Protocols

Inbunden, Engelska, 2022

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This detailed volume presents a series of protocols that are representative of recent developments and improvements in induced pluripotent stem cells (iPS cells) and corresponding human disease models.

Produktinformation

Utgivningsdatum2022-07-03
Mått178 x 254 x 30 mm
Vikt1 027 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor428
FörlagSpringer-Verlag New York Inc.
ISBN9781071625842

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- Analysis of Mitochondrial Dysfunction by Microplate Reader in hiPSC-Derived Neuronal Cell Models of Neurodegenerative Disorders. - Generation and Hematopoietic Differentiation of Mesenchymal Stromal/Stem Cell-Derived Induced Pluripotent Stem Cell Lines for Disease Modeling of Hematopoietic and Immunological Diseases. - Establishment of Human Induced Pluripotent Stem Cells from Multiple Sclerosis. - Monitoring Axonal Degeneration in Human Pluripotent Stem Cell Models of Hereditary Spastic Paraplegias Patients. - Efficient Generation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells for Disease Modeling and Disease Gene Discovery. - Autophagy Dysfunction as a Phenotypic Readout in hiPSC-Derived Neuronal Cell Models of Neurodegenerative Diseases. - Derivation of Induced Pluripotent Stem Cell (iPSC) Lines from Patient-Specific Peripheral Blood Mononuclear Cells (PBMC) Using Episomal Vectors. - Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells. - A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells. - Image-Based Quantitation of Kainic Acid-Induced Excitotoxicity as a Model of Neurodegeneration in Human iPSC-Derived Neurons. - Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures. - Creating Cell Model 2.0 Using Patient Samples Carrying a Pathogenic Mitochondrial DNA Mutation: iPSC Approach for LHON. - CRISPR Guide RNA Library Screens in Human Induced Pluripotent Stem Cells. - Generation and Encapsulation of Human iPSC-Derived Vascular Smooth Muscle Cells for Proangiogenic Therapy. - Methods to Induce Small-Scale Differentiation of iPS Cells into Dopaminergic Neurons and to Detect Disease Phenotypes. - Modeling Early Neural Crest Development via Induction from hiPSC-Derived Neural Plate Border-like Cells. - Generation of Human Induced Pluripotent Stem Cells from Renal Epithelial Cells. - A Protocol for Stepwise Differentiation of Induced Pluripotent Stem Cells into Retinal Pigment Epithelium. - Genome Editing of Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes to Model Genetic Ocular Diseases. - Generation of Cardiomyocytes and Endothelial Cells from Human iPSCs by Chemical Modulation of Wnt Signaling. - Immunoassay for Quantitative Detection of Antibody Transcytosis Across the Blood-Brain Barrier In Vitro. - Generation of Cortical, Dopaminergic, Motor, and Sensory Neurons from Human Pluripotent Stem Cells. - CRISPR/Cas-Mediated Knock-in of Genetically Encoded Fluorescent Biosensors into the AAVS1 Locus of Human-Induced Pluripotent Stem Cells. - Cancer Stem Cell Initiation by Tumor-Derived Extracellular Vesicles. - Genome Editing Using Cas9-gRNA Ribonucleoprotein in Human Pluripotent Stem Cells for Disease Modeling.