In Vitro Neurotoxicology

• In Vitro Neurotoxicology: An Introduction.- Primary Neurons in Culture and Neuronal Cell Lines for In Vitro Neurotoxicological Studies.- Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures.- Microglia Cell Culture: A Primer for the Novice.- Neural Stem Cells for Developmental Neurotoxicity Studies.- Preparation, Maintenance, and Use of Serum-Free Aggregating Brain Cell Cultures.- Neurospheres as a Model for Developmental Neurotoxicity Testing.- Acute Hippocampal Slice Preparation and Hippocampal Slice Cultures.- Glia-Neuron Sandwich Co-Cultures: An In Vitro Approach to Evaluate Cell-to-Cell Communication in Neuroinflammation and Neurotoxicity.- In Vitro Models to Study the Blood Brain Barrier.- Measurements of Cell Death in Neuronal and Glial Cells.- Measurements of Neuronal Apoptosis.- Measurement of Isoprostanes as Markers of Oxidative Stress.- Assessment of Glutathione Homeostasis.- Assessing Neuronal Bioenergetic Status.- Fluorescent Assessment of Intracellular Calcium in Individual Cells.- Homeostatic Regulation of Glutamate Neurotransmission in Primary Neuronal Cultures.- Detection of Nitric Oxide Formation in Primary Neural Cells and Tissues.- JNK3-Mediated Apoptotic Cell Death in Primary Dopaminergic Neurons.- Measurement of Proteasomal Dysfunction in Cell Models of Dopaminergic Degeneration.- Cell Signaling and Neurotoxicity: Protein Kinase C In Vitro and In Vivo.- Cell Signaling and Neurotoxicity: 3H-Arachidonic Acid Release (Phospholipase A2) in Cerebellar Granule Neurons.- Quantitative Assessment of Neurite Outgrowth in PC12 Cells.- Measurements of Astrocyte Proliferation.- Quantification of Synaptic Structure Formation in Co-Cultures ofAstrocytes and Hippocampal Neurons.- Volume Measurements in Cultured Primary Astrocytes.- Assessment of Cholesterol Homeostasis in Astrocytes and Neurons.- Dose-Response or Dose-Effect Curves in In Vitro Experiments and Their Use to Study Combined Effects of Neurotoxicants.

From the book reviews:“The variety of fresh and effective laboratory techniques makes this attractive book a necessary companion for researchers, teachers and students in this field. The editors ought to be commended for bringing together into a single, easy-access volume information on so abstruse a topic as in vitro neurotoxicology. The lay-out of the book as well as the clear and high resolution diagrams and plates are some of the most attractive attributes … .” (Jolocam Mbabazi, International Journal of Environmental Studies, Vol. 69 (6), 2012)“This volume … is a concise review of cellular, biochemical, and molecular methodological protocols for diverse areas of in vitro neurotoxicity studies. … This collection will be useful to those who are interested in studying the neurotoxicity of environmental contaminants, drugs, or other chemicals. Specifically, it will be of value to graduate students, postdoctoral fellows, and faculty in academia. … The book also provides useful methodological guidelines and examples that will be helpful to researchers who are relatively new to this field.” (Bao Ting Zhu, Doody’s Book Reviews, December, 2011)