In Vitro Mutagenesis

Methods and Protocols

Häftad, Engelska, 2018

1 569 kr

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In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions.

Produktinformation

Utgivningsdatum2018-06-27
Mått178 x 254 x 29 mm
Vikt982 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor511
FörlagHumana Press Inc.
ISBN9781493982110

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Medicinsk genetik inom Medicin

Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells.- Mutagenesis and Genome Engineering of Epstein-Barr Virus in Cultured Human Cells by CRISPR/Cas9.- Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice.- All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells.- CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-free E8 Medium.- Development of CRISPR/Cas9 for Efficient Genome Editing in Toxoplasma gondii.- Generation of Stable Knockout Mammalian Cells By TALEN-Mediated Locus-Specific Gene Editing.- Efficient Generation of Gene-Modified Mice by Haploid Embryonic Stem Cell-Mediated Semi-Cloned Technology. - Insertion of Group II Intron-Based Ribozyme Switches into Homing Endonuclease Genes.- Generating a Genome Editing Nuclease for Targeted Mutagenesis in Human Cells.- Use of Group II Intron Technology for Targeted Mutagenesis in Chlamydia trachomatis.- in silico Approaches to Identify Mutagenesis Targets to Probe and Alter Protein-Cofactor and Protein-Protein Functional Relationships.- in silico Prediction of Deleteriousness for Non-Synonymous and Splice-Altering Single Nucleotide Variants in the Human Genome.- in silico methods for Analyzing Mutagenesis Targets.- Methods for Detecting Critical Residues in Proteins.- A Method for Bioinformatic Analysis of Transposon Insertion Sequencing (INSeq) Results for Identification of Microbial Fitness Determinants.- Application of in vitro Transposon Mutagenesis to Erythromycin Strain Improvement in Saccharopolyspora erythraea.- Engineering Gram-Negative Microbial Cell Factories Using Transposon Vectors.- PERMutation Using Transposase Engineering (PERMUTE): a Simple Approach for Constructing Circularly-Permuted Protein Libraries.- Transposon Insertion Mutagenesis for Archaeal Gene Discovery.- Genome-Wide Transposon Mutagenesis in Mycobacterium tuberculosis and Mycobacterium smegmatis.- Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method.- Seamless Ligation Cloning Extract (Slice) Method Using Cell Lysates from Laboratory Escherichia coli Strains and its Application to Slip Site-Directed Mutagenesis.- Facile Site-Directed Mutagenesis of Large Constructs Using Gibson Isothermal DNA Assembly.- Revised Mechanism and Improved Efficiency of the Quikchange Site-Directed Mutagenesis Method.- An in vitro Single Primer Site-Directed Mutagenesis Method for Use in Biotechnology.- Use of Megaprimer and Overlapping Extension PCR (OE-PCR) to Site-Specifically Mutagenize and Enhance Cyclodextrin Glucosyltransferase (CGTase) Function.- Step-by-Step in vitro Mutagenesis - Lessons from Fucose-Binding Lectin PA-IIL.- Analytical Methods for Assessing the Effects of Site-Directed Mutagenesis on Protein-Cofactor and Protein-Protein Functional Relationships.- Biochemical and Biophysical Methods to Examine the Effects of Site-Directed Mutagenesis on Enzymatic Activities and Interprotein Interactions.- Use of Random and Site-Directed Mutagenesis to Probe Protein Structure-Function Relationships: Applied Techniques in the Study of Helicobacter pylori.- Novel Random Mutagenesis Method for Directed Evolution.- Random Mutagenesis by Error-Prone Polymerase Chain Reaction Using a Heavy Water Solvent.- Development and Use of a Novel Random Mutagenesis Method: in Situ Error-Prone PCR (is-epPCR).