In Vitro Fertilization

Zsolt Peter Nagy, MD, PhDScientific DirectorReproductive Biology Associates – PreludeAtlanta, GA, USAAlex C Varghese, PhDScientific DirectorAstra Fertility GroupMississauga, ON, CanadaAshok Agarwal, PhD, HCLD (ABB), EMB (ACE),American Center for Reproductive MedicineCleveland ClinicCleveland, OH, USA

• Journey of Human Gametes In Vitro--1978 to 2018.- Setting up an ART unit - Planning, Design and Construction.- Building the Laboratory.- Air Quality Management.- Organization And Workflow In A Fertility Clinic.- Risk and Safety in the IVF Clinic.- Control of variables.- Daily, Weekly, and Regular Preparations for the IVF Laboratory.- Essential Instruments and Disposable Supplies for an IVF Laboratory.- Co2 and Low-O2 Incubators.- What to consider when selecting a LAF, Class II Cabinet or Isolette for your ART program?.- Culture Media in IVF: Decisions for the Laboratory.- Assessment Of Oocyte Quality.- Oocyte Denuding.- Interrupted or Uninterrupted Culture to the Blastocyst Stage in a Single Medium.- Sequential Media for Human Blastocyst Culture.- In Vitro Maturation of Human Oocytes.- Design and Development of Simplified, Low Cost Technologies for Clinical IVF: Applications in High and Low Resource Settings.- In vitro culture of oocytes and embryos with micro-vibration: “naturalization” of routine IVF procedure.- Microfluidics for Gamete Manipulation and Embryo Culture.- Time-Lapse Microscopy for Embryo Culture and Selection.- Sperm Assessment: Traditional Approaches and Their Indicative Value.- Sperm Assessment: Novel Approaches and Their Indicative Value.- Assessment of Sperm Chromatin Damage by TUNEL Method Using Bench Top Flow Cytometer.- Sperm processing in ART.- PESA/MESA/TESA/TESE Sperm Processing.- Processing Sperm Samples in HIV-Positive Patients.- An Emerging Medical Device: Electrophoretic Sperm Separation.- Magnetic Activated Cell Sorting of Human Spermatozoa.- Intrauterine Insemination.- Conventional IVF Insemination.- Intra-Cytoplasmic Sperm Injection.- ntracytoplasmic Morphologically Selected Sperm Injection.- Intracytoplasmic Morphologically Selected Sperm Injection.- Oocyte Activation Deficiency and Advances to Overcome.- Hydraulic Manipulators for ICSI.- Research Instruments Micromanipulation Systems .- Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators.-Oocyte Treatment and Preparation for Microinjection.- Piezo-ICSI.- Traditional Embryo Morphology Evaluation: From the Zygote to the Blastocyst Stage.- Predicting Embryo Implantation Potential Using Video Monitoring By the Embryoscope Time-Lapse System.- Predicting Embryo Developmental Potential and Viability Using Automated Time-Lapse Analysis.- Proteomics and Metabolomics.- Amino Acid Turnover as a Biomarker of Embryo Viability.- Microfluidics and Microanalytics to Facilitate Quantitative Assessment of Human Embryo Physiology.- Assisted Hatching of Human Embryos for Successful Implantation.- Polar Body Biopsy.- Cleavage-Stage Embryo Biopsy.- Current and Novel Methods for Chromosome Testing.-Embryo Biopsy forPGD: Current Perspective.- Sperm Cryopreservation.- Technologies of Cryoprotectant-free Vitrification of Human Spermatozoa: Asepticity as Criterionn of Effectiveness.- Slow Freezing of Oocytes.- Vitrification: Methods Contributing to Successful Cyopreservation Outcomes.- Oocyte Vitrification and Current Clinical Applications.- Slow Freezing of Embryos.- Vitrification of Embryos.- Ovarian Tissue Cryopreservation.- Establishing and Managing Donor Oocyte Banking.- Single Embryo Transfer.- Embryo Transfer:  Techniques and Trouble shooting.- G-CSF and GM-CSF: Clinical applications in reproductive medicine.- The Freeze All Cycle: A New Paradigm Shift in ART.- IVF Data Management:  From Clipboards to Smart Apps.- Regulation of the ART Laboratory.- Legislation in the UK.- Regulation, Licensing, and Accreditation of the ART Laboratory in Europe.- Regulation, Licensing, and Accreditation of the ART Laboratory in Brazil.- Regulation, Licensing and Accreditation of ART Laboratories in Australia.- Trouble Shooting In Ivf Laboratories.- Benchmarking and Decision Making in the IVF Laboratory.- Automation, Artificial Intelligence and innovations in the future of IVF.- Assisted reproductive technologies to prevent transmission of mitochondrial DNA disease.- Nuclear and Cytoplasmic Transfer: Human Applications and Concerns.- Embryo culture and phenotype of the offspring.- Traceability in ART.- The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development.- Cytoskeletal architecture of human oocytes with a focus on centrosomes and their significant role in fertilization.- Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence.- Livestock Production via Micromanipulation.