In Situ Hybridization

Principles and Practice

Inbunden, Engelska, 1999

Av Julia M. Polak, James O'D. McGee, London) Polak, Julia M. (, Department of Histochemistry, Royal Postgraduate Medical School, Oxford) McGee, James O'D. (, University of Oxford Nuffield Department of Pathology and Bacteriology, John Radcliffe Hospital, Julia Polak, James McGee

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This practical guide covers all aspects of in situ hybridization, describing: practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology. This new edition has been completely rewritten by the experts in the field to take into account the many advances in the last seven years.

Produktinformation

Utgivningsdatum1999-01-28
Mått159 x 240 x 17 mm
Vikt507 g
FormatInbunden
SpråkEngelska
Antal sidor224
Upplaga2
FörlagOUP OXFORD
ISBN9780198548805

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Biologi inom Naturvetenskap och teknik
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1. Principles of in situ hybridization ; 2. The design and generation of probes for in situ hybridization ; 3. The preparation of non-radioisotopic hybridization probes ; 4. Principles and applications of complementary RNA probes ; 5. Quantification of in situ hybridization ; 6. Quantification of grains in in situ hybridization ; 7. Detection of genetic changes in cancer by interphase cytogenetics and comparative genomic hybridization ; 8. Single and simultaneous nucleic acid detection in clinical samples ; 9. Combined in situ hybridization and immunocytochemistry: investigation of viral pathogenesis at the light and electron microscope levels ; 10. Super sensitive in situ DNA and RNA localisation

Anyone contemplating using in situ hybridisation in their laboratory will need a thorough grounding in the background and principles of the methods. This book excels in its background presentation, with clear explanations and good referencing.....There are very fewbooks dealing with the practicalities of in situ hybridisation. This one makes a major contribution to the subject and therefore is invaluable as a reference for many scientists and clinicians Dr Deborah K Dun-Waters, The Bulletin of the Royal College of Pathologists, No. 107 June 1999