Immunology of Infection

Dieter Kabelitz is full professor at the University of Kiel, Germany, and Director of the Institute of Immunology. Dieter Kabelitz studied medicine in Freiburg and Munich, Germany. He was a post-doc in Uppsala/Sweden and at Rockefeller University, New York. In 1988, he was appointed Associate Professor for Cellular Immunology at the University of Heidelberg. From 1992 to 1999 he was Head of the Department of Immunology at the Paul-Ehrlich Institute in Langen, Germany. Since 1999 he is a full professor at the University of Kiel and Director of the Institute of Immunology. His scientific expertise is in the field of the functional characterization of human T cells with a special focus on  T cells. Current projects aim to explore the potential of human  T cells for the immunotherapy of cancer. He has published more than 360 papers listed in PubMed/Medline. Dieter Kabelitz was chairman of the Collaborative Research Center (SFB) 415 „Specificity and Pathophysiology of Signal Transduction Pathways“ funded at Kiel University by the German Research Foundation (DFG). He is a founding member and member of the Steering Committee of the DFG-funded Cluster of Excellence „Inflammation-at-Interfaces“. He was President of the German Society for Immunology (DGfI) in 2011 and 2012, and he is a Council member of the International Union of Immunological Societies (IUIS) and chair of the Education Committee (EDU) of IUIS.

• IntroductionThe immune response to infectious agentsGeneral MethodsPhenotyping and separation of leukocyte populations based on affinity labellingCytometric cytokine secretion assay: detection and isolation of cytokine secreting T cellsMeasurement of cellular proliferationUse of bioinformatics to predict MHC ligands and T-cell epitopes: application to epitope driven vaccine designThe use of tetramers in the quantitative analysis of T cell responsesUsing microarrays for studying the host transcriptional response to microbial infectionGenome-wide expression profiling of intracelllular bacteria: the interaction of Mycobacterium tuberculosis with macrophagesMurine ModelsManagement of immuno-compromised and infected animalsIn Vitro AnalysisIsolation and preparation of lymphocytes from infected animals for in vitro analysisIsolation and development of murine T-lymphocyte hybridomas and clonal cell linesKiller cell assaysQuantitation of T Cell Cytokine Responses by ELISA, ELISPOT, Flow Cytometry and Reverse Transcriptase-PCR MethodsIsolation of and measuring the function of professional phagocytes: murine macrophagesIn vivo AnalysisThe immune response in mice challenged with bacterial infectionsMeasuring immune responses in vivoSpecific ModelsMurine model of tuberculosisThe leishmaniasis modelAnimal models: murine cytomegalovirusDNA vaccines: fundamentals and practicePreparation and use of adjuvantsHuman SystemsIsolation and propagation of human dendritic cellsIsolation of T cells and establishment of T-cell lines and clonesGrowth-transformation of human T cellsGeneration and characterisation of human killer cellsMeasuring human cytokine responsesMeasuring immune responses in situ: immunofluorescent and immunoenzymatic techniquesIsolation, characterisation and cultivation ofhuman monocytes and macrophages

Praise for the first edition: "This is a useful compendium of methods and techniques for those wishing to examine the immune responses to infections in vitro and in vivo. The book is clearly presented, packed with protocols, and well illustrated" --Microbiology Today "...it represents a valuable addition to the microbiologists' reference source" --Immunology Today Praise for the Series:"An invaluable resource for the detailed analysis of cellular gene function and genome architecture." --NATURE