Immunological Methods in Microbiology

Dr Gurtler completed a Ph.D. program in 1996 at La Trobe University in the Department of Microbiology. The main contribution of this work was the development of a universal bacterial typing technique based on the 16S-23S rDNA spacer region. The technique is now extensively used in many areas of Microbiology including Diagnostic, Environmental and Veterinary Microbiology. Citations to the articles Dr Gurtler wrote on this topic total >1000 with >830 citations alone to the 1996 review published in the journal “Microbiology”. Dr Gurtler wrote an invited review on genomic typing, taxonomy and identification of bacterial isolates for the International Journal of Systematic and Evolutionary Microbiology (IJSEM). In addition to fundamental research, Dr Gurtler has also had experience with the application of this technology to the medical diagnostic laboratory in the fields of molecular microbiology and human molecular genetics. Dr Gurtler has been involved full time in diagnostic microbiology over many years resulting in the adoption of a method that identifies Mycobacterium species directly from clinical specimens without the need for culture in specimens positive for acid fast bacilli, the discovery of Nocardia veterana, and the development of many diagnostic tests.In the last twelve years Dr Gurtler has been an Editor for the Journal of Microbiological Methods (JMM) and in the last 5 years Serial Editor of Methods in Microbiology (MIM) with the publication of 9 volumes covering diverse subjects such as Biofilms, Nanotechnology, COVID-19 and Fluorescent Probes.Dr. Pavia is a Microbiologist/Immunologist and an Associate Professor at the NYIT College of Osteopathic Medicine in Old Westbury, New York. He is also an Adjunct Associate Professor at New York Medical College. He previously worked and held research appointments at the Trudeau Institute in Saranac Lake, NY, and the Walter Reed Army Institute for Research in Washington, DC. Throughout his career, he has focused his research activities on various pathogenic microbes with a particular emphasis on the disease-causing spirochetes and certain protozoan pathogens. His research has covered multiple areas including the basic microbiologic, immunologic, molecular, diagnostic and clinical aspects of Lyme disease, syphilis, malaria and toxoplasmosis. He has published widely on these areas in peer-reviewed journals, as well as given numerous research presentations of his work at national and international conferences. As a principal investigator or co-investigator, he has received research funding from the NIH, the CDC, the J.M. Foundation, and various other organizations to support his research work. He has been a member of the editorial board of the Journal of Microbiological Methods for the past 25 years, and has been an ad-hoc reviewer for a wide range of other scientific journals.

• 1. Introduction to immunological techniques in the clinical laboratoryTony A. Slieman and Joerg Leheste2. Combining antigen detection and serology for the diagnosis of selected infectious diseasesMaria M. Plummer and Charles S. Pavia3. Immunologic detection of Lyme disease and the related borreliosesCharles S. Pavia4. Immunodetection of bacteria causing brucellosisSukhadeo B. Barbuddhe, Jess Vergis and Deepak B. Rawool5. Immunological and molecular techniques used for determination of serotypes in PasteurellaceaeHenrik Christensen, Janine Bossé, Øystein Angen, Niels Nørskov-Lauritsen and Magne Bisgaard6. Immunological tests for diarrhoea caused by diarrhoeagenic Escherichia coli targeting their main virulence factorsRoxane M.F. Piazza, Bruna A. Caetano, Camila P. Henrique, Daniela Luz, Danielle D. Munhoz, Juliana M. Polatto, Letícia B. Rocha, Miriam A. Silva and Thais Mitsunari7. Development and improvement of a colony blot immunoassay for the detection of thermotolerant Campylobacter speciesHongsheng Huang, Philippe Raymond, Chris Grenier and Jessica Fahey8. Serodiagnosis of Strongyloides stercoralis infectionRahmah Noordin, Norsyahida Arifin, Dinesh Balachandra and Hussain Ahmad9. Immunological detection of Zika virus: A summary in the context of general viral diagnosticsRebecca Peters10. Detection of microorganisms using recombinase polymerase amplification with lateral flow dipsticksYuhang Zhang, Jinqiang Hu, Qingmei Li, Junqing Guo and Gaiping Zhang11. Detection of microorganisms with lateral flow test stripsTatsuya Tominaga and Masaharu Ishii