Immunoassays

Dr. Arnold Maria Raem works at the Centre for Nanotechnology at Westfälische Wilhelms University Münster, Germany, in the field of molecular biomedicine/oncology.Dr. Peter Rauch is Managing Director of Candor Bioscience GmbH in Germany, a biotechnology company involved in the research and development of products for immunoassays.

• Forewords.- Introduction to Immunoassays.- Antibodies.- ELISA/EIA/FIA.- Sample Preparation for Immunoassays.- Lateral Flow Immunoassays.- Immuno-PCR: Application-oriented highly sensitive protein analytics with antibody-DNA conjugates.- Evolution of PCR – from classical PCR to digital PCR.- Detection of Proteins on Membranes: Western Blot and Dot Blot Methods.- Acceleration of Western Blotting.- Lab-on-a-Chip – Miniaturization of Assays for On-Site Diagnostic Applications Using Microfluidics.- Reliable protein quantification by surface plasmon resonance spectroscopy.- Troubleshooting and Assistance in the Development, Validation and Implementation of ELISAs.- Detection Methods for Protein Interaction.- Immunohistochemistry for Research and Diagnostic Pathology.- Fluorescence Microscopy in Immunoassay Application – An Introduction.- In Vivo Immunostaining and Intravital Two-Photon Microscopy.- Quantitative digital holographic phase contrast microscopy – biophysical characterization of immunological processes, cells and tissues.- Circulating Tumor Cells – Isolation and Molecular Characterization.- Automated isolation of circulating tumor cells for liquid biopsy.- 3D Cell and Organoid Culture.- Tissue Engineering and Regenerative Medicine.- The CRISPR/Cas Technology: Classic and Future Applications in Molecular Biology and Medicine.- Fluorescent Dyes.- Stabilization of Assay Components.- Interference Effects in Immunoassays.- Troubleshooting and Assistance in the Development, Validation and Implementation of ELISAs.- Evaluation and Validation.- Food and Feed Analysis.- Automation of Immunoassays.- Challenges for a Next Generation Biobanking – Cryopreservation for the advancement of personalized medicine.- The new EU Regulation 2017/746 on in vitro diagnostic medical devices.- ISO 13485 – the mandatory quality system for IVD manufacturers.- Clinical Trials for In-vitro Diagnostics.- Protection for Diagnostic Methods and its Limits under the European Patent Convention and the German Patent Law.- Learning Laboratory Systems: How can artificial intelligence support laboratory work?.- Impedance-based Analysis of Functional Immunoassays using Cultured Cells.- Measurement of Single Molecules and Cells with Atomic Force Microscopy.- Immunoassays for Extracellular Vesicles-based Characterization.- Immunoassay Fabrication and Device Functionalization by Scanning Probe Lithography.- The digital lab of the future, laboratory information systems, analytics and AI in LifeScience R&D.- Regulation of Medical Devices in the USA with a Focus on IVDs.- Optical Coherence Tomography for Non-Invasive Imaging of Tissue and Organoids.- sFIDA: Quantitative Single Particle Analysis.- The Blood-Brain Barrier.- The Biomedical Lab, Ethics, and Obtaining Ethics Approval for Research.- A label-free future: digid´s BioMEMS platform enables diagnostics beyond the laboratory.- Ethical Boundaries of Research Freedom.- New Approaches in Personalized Tumor Therapy Research: Organoids, Organoid-on-Chip, and Tumor Explants as Models.

Select Guide Rating